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Download Symphony User Manual - Protein Technologies, Inc.
Transcript
• • • • • • • • • • • Pressure and prime Sol 2, Sol 3, Sol 4, Sol 6 as required and look for leaks. Backflush S1 (Flush Solv button) for all 20 AA lines and verify all get liquid to them. Press bottles (leave sit 5 minutes), do pressure test. Go to manual screen and assign S1 and run all RV's. Verify liquid delivery and drain into waste 1. Unplug waste 1 connector and repeat operation and verify liquid delivery and drain into waste 2 (check for leaks on waste valve fittings). Load AA bottles for test peptide run. Pressure AA's and check for leaks. Prime AA's (2X). Load resins for run. Load pep files to RV positions, start synthesis. Assign cleavage programs to RV's with appropriate start times. APPENDIX B: HANDLING AND SHELF LIFE OF REAGENTS CAUTION - THIS INSTRUMENT CONTAINS SOLVENTS AND CHEMICALS THAT SHOULD BE HANDLED CAREFULLY. MANY ARE EASILY ABSORBED THROUGH THE SKIN AND CAN CAUSE ADVERSE HEALTH EFFECTS. WEAR SAFETY GLASSES, PROTECTIVE CLOTHING AND RUBBER GLOVES AT ALL TIMES. FOLLOW MSDS HANDLING GUIDELINES PROVIDED WITH THE INDIVIDUAL REAGENTS. RESPIRATORS AND ABSORBENT SHOULD BE AVAILABLE IN THE EVENT OF A SPILL. B-1 Reagent Shelf Life Proper handling and storage of peptide synthesis reagents is important to the successful performance of your instrument. Please review the table on the following pages and make certain that your reagents are correctly stored. Be sure to rotate your stock of reagents using "first in, first out" so that their shelf-life is not exceeded before use. Description Temperature Condition Shelf Life Amino Acids Amino Acids SOLV1 = N,NDimethylformamide DMF DEP = Deprotectant 20% (V/V) Piperidine in DMF SOLV2 = Methylene Chloride (DCM) CAP = Capping Solution Acetic anhydride ACT = Activating solution (*) 100mM HBTU/0.4 M 4-Methy lmorpholine in DMF CLEAV = Cleavage reagent** 100% Trifluoroacetic acid CLEAV = Cleavage reagent with scavengers*** Room Temperature* Room Temperature* Room Temperature* Dry Dissolved in DMF ----- Stable 14 days Stable Room Temperature* ----- Stable Room Temperature* ----- Stable Room Temperature* ----- Stable Room Temperature* ----- 5 - 7 days Room Temperature* ----- Stable Room Temperature* ----- 2 days 98
