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To set the Preprocess Parameters: Step 5 (continued) Action Click OK when done. Note The matrix file must be created previously and saved to the ABI folder, which is located in the System folder. Refer to Appendix B in the 373 DNA Sequencing System User’s Manual for instructions on making a matrix. Be sure to use the Dye Primer Matrix Standard Kit (P/N 401114) and Filter Set A. To set the Analysis Parameters: Step Action 1 Choose Analysis Parameters from the Analysis menu. 2 Set the parameters as shown below. 3 Click the Save Settings button. 4 Click OK when done. 5 Quit the GeneScan 672 Fragment Analysis Software. continued on next page 7-20 SSCP Analysis