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Transcript
Chapter 6
Slide Dispensing
In this page users can customize how many arrays will be printed on the slide, how many
spots will be in an array, and how the arrays and spots are arranged. Users can preview the
arrangement on a virtual slide in the box on the right.
Spot-to-spot distance within an array in μm. It is dependent upon the diameter
Spot
of the spot, usually 800μm - 1500μm.
Distance
The number of deposition action taken by the Dispenser; specifically the
Spots Count
number of spots printed by the Dispenser within an array. It should not be
per Channel
greater than the Max. Spot Count.
The maximum number of spots is mainly dependent upon the dimensions of
Max. Spot
the printable area and the Spot Distance.
Count
Max. Spot Count is determined by the Spot Distance and the PX/PY.
Refers to how many replicates are required for a single sample. It should not be
Replicate(s)
greater than the product of the values of the Spots Count per Channel along
per Sample
the X axis and the Y axis.
The distance between the edges of two neighboring arrays. It should not be less
Array
than the Spot Distance.
Separation
Refers to how many arrays will be arranged on the slide (along each axis). It
Array
must not be greater than the Max. Array Count.
Count
Max. Array It is determined by Array Separation, the size of the array and the printable
area.
Count
Array
Indicates whether the arrays share the same sample sequence.
Replication
PreSpot count per sample for Pre-dispensing, ranging from 0~500. Predispensing
dispensing is necessary to ensure the uniformity of spots on the slide.
Count
PreThe duration of the dispensing during the Pre-dispensing step will affect the
dispensing
volume delivered. The unit is μs, ranging from 10μs ~2500μs.
Time
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PersonalArrayerTM 16