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Using Tubes, Bottles, and Accessories THREE-PIECE CAP ASSEMBLIES Cap bottles with three-piece cap assemblies as follows: Cap Plug O-ring Bottle Lip 1. Be sure the O-ring, plug, and bottle lip are dry and free of lubrication. 2. Place the O-ring on the underside of the plug. 3. Insert the plug into the neck of the bottle, ensuring that no fluid contacts the O-ring. 4. Tighten the cap by hand. JLA-8.1000 AND JLA-9.1000 BOTTLE CAP/CLOSURES Tighten the cap/closure until this mark aligns with or goes past the bottle vent line MAX FILL LINE Place the plug on the bottle, then screw on the cap/closure by hand as tightly as possible. Tighten until the timing mark on the cap/closure is aligned with or goes past the vent line on the bottle. 1000mL Vent Line FILLING AND LOADING CUPS IN THE JS-5.0 ROTOR ➠ NOTE Four labware cups must be used for every run and must be balanced to within 25 grams of each other. Do not load the rotor with two filled cups and two empty cups. 1. Insert four labware cups into two cup racks with the cup latch hinges toward the center of the racks. 2. Make sure that the gaskets and sealing surfaces on each cup and cover are clean and dry. Place a gasket around the top edge of each cup, carefully pushing the gasket down until it is fully seated on 3-8 J Series Rotors and Tubes