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Chapter 5 – Experiment Manager
Properties, Kinetic
Use ROIs From Image. The ROIs have to be drawn into a snapshot (or any image of the same XY
dimension as the image to be acquired) before the start of the experiment. Choose this snapshot
from the list in this box.
Z Stack layer. In a 3-D Time-Lapse series only one Z-layer can be chosen for the online calculation
of a kinetic. Set this layer here.
Stage Position. An online kinetics analysis is possible only for one of the stage positions in an
experiment to be carried out at different positions. Set this position here.
Properties, Store
If the option Store is marked – this is indicated by the storage symbol in the top right corner of the
icon – the kinetics graph that is being calculated online will be stored on the hard disk after the
experiment has been finished otherwise it will be trashed.
The display of the online kinetics is automatic and does not need to be activated as in
case of the online ratio image calculation. It is possible to analyze several image sets in
one experiment (in other words, to connect Online Kinetics commands to several Image Acquisition commands in one Experiment Plan). However, same as for the offline
analysis, only one graph (with several ROI curves) of one image set can be displayed at
a time. The graph to be displayed can be chosen and changed in the Graph gallery of
the Image Manager box at the left side of the user interface during the experiment.
The calculation of a kinetics analysis is a time consuming command for the PC processor. The software estimates the additional CPU usage and might change the minimal
cycle in dependence of online analyses. However, in rather fast and complex experiments it still may happen that the image acquisition is faster than the generation of
online analysis results by the PC. In such a case the image acquisition timing has the
highest priority and the analysis will remain incomplete. That means, while certain data
points will be missing in the graph, all images will be acquired as designed in the Experiment Plan and stored in the database. The analysis has then to be repeated offline.
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