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TABLE OF CONTENTS
ESSENTIAL SAFETY NOTES ....................................................................................................................................................... 3
Unpacking, Positioning and Installation ...................................................................................................................... 3
INTRODUCTION ............................................................................................................................................................................ 4
Your spectrophotometer ............................................................................................................................................... 4
Sample handling tips ..................................................................................................................................................... 4
Keypad and display ....................................................................................................................................................... 8
Software style............................................................................................................................................................... 10
THE APPLICATIONS FOLDER ................................................................................................................................................... 11
1: Single Wavelength – Abs and %T .......................................................................................................................... 12
2: Concentration .......................................................................................................................................................... 14
3: Wavescan ................................................................................................................................................................. 16
4: Simple Kinetics ........................................................................................................................................................ 19
5: Standard Curve ........................................................................................................................................................ 22
6: Multiple Wavelength ................................................................................................................................................ 26
7: Absorbance Ratio .................................................................................................................................................... 27
THE LIFE SCIENCE FOLDER..................................................................................................................................................... 29
1: DNA ................................................................................................................................................................... 30
2: RNA ................................................................................................................................................................... 32
3: Oligo .................................................................................................................................................................. 34
Protein Determination ................................................................................................................................................. 36
1: Protein UV ......................................................................................................................................................... 37
2: BCA ................................................................................................................................................................... 39
3: Bradford............................................................................................................................................................. 42
4: Lowry ................................................................................................................................................................. 45
5: Biuret ................................................................................................................................................................. 48
Bacterial Cell Culture Measurement (OD600) ............................................................................................................ 51
FAVOURITES AND METHODS FOLDERS ................................................................................................................................ 53
UTILITIES FOLDER..................................................................................................................................................................... 54
Utilities .......................................................................................................................................................................... 55
1: Date and Time ................................................................................................................................................... 55
2: Regional ............................................................................................................................................................ 55
3: Printer ................................................................................................................................................................ 56
4: Preferences ....................................................................................................................................................... 57
5: Contrast ............................................................................................................................................................. 57
6: Folder Names .................................................................................................................................................... 57
7: About ................................................................................................................................................................. 58
8: Games ............................................................................................................................................................... 58
ACCESSORIES INSTALLATION ................................................................................................................................................ 60
Printer installation ....................................................................................................................................................... 60
Loading / changing the printer paper ......................................................................................................................... 62
Bluetooth accessory installation ................................................................................................................................ 63
SD Card accessory installation and operation .......................................................................................................... 63
PRINT VIA COMPUTER .............................................................................................................................................................. 74
ACCESSORIES ........................................................................................................................................................................... 75
After Sales Support ..................................................................................................................................................... 75
Lamp Replacement ...................................................................................................................................................... 75
Cleaning and general care of the instrument ............................................................................................................ 76
SPECIFICATION AND WARRANTY ........................................................................................................................................... 77
Version 5.1 21/3/2011
Page 2
Picodrop Ltd.