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PDQuest User Guide Step 3: Place a clean plastic Gel Sheet on top of the gel Step 4: Place the microtiter plate on top of the plastic sheet. Note the orientation so that you can recognize the A1 location. Step 5: Close the Enclosure door Step 6: Click on "Acquire image" when the plate is in place. The UV lights will turn on and the camera will acquire an image with the same exposure time as the first spot cutter image. Step 7: Assess the image on the screen for the wells that need to be recut. Fig. 8-16. Microtiter plate with fluorescent spots in wells. Use the Zoom and transform functions on the right side of the "Image Microtiter Plate" window to get a better view of the microtiter plate wells. 8-20
