Download RNA-Seq Legacy

Transcript
Contents
1 Using the Legacy RNA-Seq Plugin
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1.1 Introduction . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .
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1.2 Specifying reads, reference genome and mapping settings . . . . . . . . . . . . .
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1.2.1 Paired data in RNA-Seq . . . . . . . . . . . . . . . . . . . . . . . . . . . .
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1.2.2 Finding the right reference sequence for RNA-Seq . . . . . . . . . . . . . .
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1.3 Exon identification and discovery . . . . . . . . . . . . . . . . . . . . . . . . . . .
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1.4 RNA-Seq output options . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .
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1.4.1 RNA-Seq report
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1.4.2 Gene fusion reporting . . . . . . . . . . . . . . . . . . . . . . . . . . . . .
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1.5 Interpreting the RNA-Seq analysis result . . . . . . . . . . . . . . . . . . . . . . .
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1.5.1 Gene-level expression . . . . . . . . . . . . . . . . . . . . . . . . . . . . .
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1.5.2 Transcript-level expression . . . . . . . . . . . . . . . . . . . . . . . . . .
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1.5.3 Definition of RPKM
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2 Installation of the Legacy RNA-Seq Plugin
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3 Uninstall
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Bibliography
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