Download RNA-Seq Legacy
Transcript
Contents 1 Using the Legacy RNA-Seq Plugin 4 1.1 Introduction . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 4 1.2 Specifying reads, reference genome and mapping settings . . . . . . . . . . . . . 5 1.2.1 Paired data in RNA-Seq . . . . . . . . . . . . . . . . . . . . . . . . . . . . 8 1.2.2 Finding the right reference sequence for RNA-Seq . . . . . . . . . . . . . . 9 1.3 Exon identification and discovery . . . . . . . . . . . . . . . . . . . . . . . . . . . 9 1.4 RNA-Seq output options . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 10 1.4.1 RNA-Seq report . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 12 1.4.2 Gene fusion reporting . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 14 1.5 Interpreting the RNA-Seq analysis result . . . . . . . . . . . . . . . . . . . . . . . 15 1.5.1 Gene-level expression . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 15 1.5.2 Transcript-level expression . . . . . . . . . . . . . . . . . . . . . . . . . . 17 1.5.3 Definition of RPKM 19 . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 2 Installation of the Legacy RNA-Seq Plugin 20 3 Uninstall 22 Bibliography 23 3