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CHAPTER 14. RESTRICTION SITE ANALYSES
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Figure 14.12: Selecting number of cut sites.
• N restriction sites
Minimum
Maximum
• Any number of restriction sites > 0
The default setting is to include the enzymes which cut the sequence one or two times.
You can use the checkboxes to perform very specific searches for restriction sites: e.g. if you
wish to find enzymes which do not cut the sequence, or enzymes cutting exactly twice.
14.2.3
Output of restriction map analysis
Clicking next shows the dialog in figure 14.13.
Figure 14.13: Choosing to add restriction sites as annotations or creating a restriction map.
This dialog lets you specify how the result of the restriction map analysis should be presented:
• Add restriction sites as annotations to sequence(s). This option makes it possible to see
the restriction sites on the sequence (see figure 14.14) and save the annotations for later
use.