Download Prelude User Manual - Protein Technologies, Inc.

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may be installed and left in place throughout several sets of syntheses.
This solvent is utilized in the automated cleaning operations for the valve
fluid system. Therefore, SOLV 1 must be in place for normal operation of
the instrument. This solvent position is also utilized during the Bottle
Position Flush (Section 2.5.3.2), Rinse All Blocks (Section 2.5.3.6) and
Wash RVs (Section 2.5.3.8) cleaning operations.
SOLV 2 or DCM: 1 L safety-coated glass bottle for a secondary wash
solvent, such as DCM to wash the peptide-resin in preparation for
automated cleavage. This bottle position is utilized during the System
Clean (Section 2.5.3.1), Cleave Bottle Solvent Back Flush (Section
2.5.3.3), and Collect Back Flush (Section 2.5.3.5) cleaning operations.
DCM may be installed and left in place for several sets of syntheses.
SOLV 3 or Dep: 1 L safety-coated glass bottle for deprotectant to remove
the N-terminal Fmoc protecting group. The standard composition is 20%
(v/v) piperidine in DMF. This solution is also quite stable and may be
installed and used for several sets of syntheses. Other reagents may be
loaded for alternate chemistries.
SOLV 4 or Cap: 1 L safety-coated glass bottle for capping solution to
permanently block any unreacted amino groups following a coupling
reaction or to acetylate the N-terminus of a completed peptide. Typical
compositions include 1:1:3 acetic anhydride/pyridine (or DIPEA)/DMF.
Other reagents may be loaded for alternate chemistries.
SOLV 5 or Base: 1 L safety-coated glass bottle for base, if separation of
base and coupling reagent is desired. The standard base composition is
0.4 M NMM in DMF. Bottle 6 or 7 should then contain a coupling reagent
such as 0.1 M HBTU in DMF. Alternatively, base and coupling reagent
may be combined into a single activator solution as described below.
SOLV 6 or Act1: 1 L safety-coated glass bottle for activator solution to
form the activated Fmoc amino acid for the coupling reaction. The
standard composition is 0.1 M HBTU in 0.4 M NMM in DMF. Other
reagents may be loaded for alternate chemistries.
NOTE The activator solution must be equimolar with the amino acid
solutions. The activator solution should be prepared fresh for each
synthesis.
SOLV 7 or Act2: 1 L safety-coated glass bottle for additional reagent.
SOLV 8 or CLEAV: 1 L safety-coated glass bottle of cleavage reagent for
cleavage of the peptide from the resin after synthesis is complete. This
position is specifically designed to handle the aggressive TFA cleavage
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