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they relate to the resin drying operation. As an example, after the final SOLV 5 rinse
(methylene chloride) prior to cleavage, the resin is usually dried by blowing nitrogen. The
DRY ON field specifies the duration of intermittent blowing of nitrogen in from the top of the
reaction vessel. DRY OFF specifies the duration the blowing is to be off. The default settings
are 10 seconds for DRY ON and 20 seconds for DRY OFF. Thus, throughout a programmed
10-minute drying operation, the nitrogen will alternate blowing into the reaction vessel for 10
seconds and then stop for 20 seconds throughout the 10-minute drying cycle. The minimum
and maximum values for these fields is 0 and 40.
The CLVOFF time is similar to the MIX OFF time. During the mix step in a cleavage, the MIX
ON time during which nitrogen is bubbled through the resin is FIXED at 1 second. The only
option is to vary the OFF value. This is done with the CLVOFF time. The default setting is
120 seconds, i.e. throughout the cleavage mix time nitrogen will agitate the resin for one
second and then be off for 120 seconds. The minimum CLVOFF time is zero seconds and
the maximum is 200 seconds. The relatively long CLVOFF minimum time is due to the
volatile nature of TFA cocktails and the generally long times assigned to the cleavage
process.
3.1.1 Command Buttons
3.1.1.1 Send
The Send button sends the currently open operation time’s file to the Symphony
instrument to execute these operation times.
3.1.1.2 Defaults
The Default button loads the Default values introduced at the factory.
3.1.1.3 Close
The Close Button exits the current screen to the main menu screen. If the operator
has made any changes to the file, a Save Message Window appears asking if you would like
to save the changes. If the operator presses Yes, then an Operation Times File Save Dialog
box appears and now the operator can save the file. Operation Times files are saved with a
.opt file extension.
3.2
Reagent (AA) Data
The Reagent (AA) Data option is available through the SetUp menu on the Main Menu
Bar. This opens the Reagent (AA) Data editor, which is a spreadsheet for setting formula
weights of the reagents or amino acids, both in its deprotected and protected forms. A
Reagent Data file is used by the Peptide/Sequence Editor and the Calculations and Calculate
Yields screens and indirectly in the Good Laboratory Practice Files. Since a GLP file is
specified in the Bottle Preparation screen, these Reagent data files are indirectly used for all
RV Operations. Reagent Data files are saved with a .aa file extension.
There are six columns on the Reagent (AA) Data screen shown below. The first one has Pos
for Position as title and below it the numbers one to twenty (indicating bottle position). Next is
the Key column where a one-letter abbreviation is used to identify the reagent (amino acid).
Next is a three-letter abbreviation of the name of the reagent (amino acid). The fourth column
is used for the complete description of the reagent (amino acid). Then is the column for the
Deprotected Molecular Weight and last the one for the Protected Molecular Weight.
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