Download Automated DNA Sequencing
Transcript
Figure 7-4 Raw data from the sample run in lane 29 of the gel in Figure 7-1 on page 7-4 If analysis fails, the raw data is the default window that appears when opening the sample file. Figure 7-5 shows a failed BigDye terminator reaction on the ABI PRISM 310 Genetic Analyzer. Only unincorporated dye terminator peaks are seen. Figure 7-5 Raw data from a failed reaction Figure 7-6 shows raw data from a successful BigDye terminator reaction on the ABI PRISM 310 Genetic Analyzer. Figure 7-6 Raw data from a successful reaction Data Evaluation and Troubleshooting 7-7