Download WINWCP User Guide - Spider

Transcript
Table of Contents
Introduction
Welcome ..................................................................................................................................................... 4
Main Features of WinWCP ........................................................................................................................ 5
Conditions of Use ...................................................................................................................................... 7
Getting Started
Hardware Requirements........................................................................................................................... 8
Installing WinWCP..................................................................................................................................... 9
Laboratory Interfaces
National Instruments Interface Cards................................................................................................ 10
Axon Instruments Digidata 1200....................................................................................................... 15
Cambridge Electronic Design 1401 Series........................................................................................ 18
Axon Instruments Digidata 1320....................................................................................................... 21
Molecular Devices Digidata 1440A..................................................................................................... 23
Molecular Devices Digidata 1550/1550A .......................................................................................... 26
Instrutech ITC-16/18 ........................................................................................................................... 28
Biologic VP500 ...................................................................................................................................... 30
Tecella Pico/Triton/Triton+ ................................................................................................................ 31
Heka Patch Clamps & Interfaces ........................................................................................................ 32
Amplifiers
Patch/Voltage-clamp Amplifiers ......................................................................................................... 34
Signal Connections Table.................................................................................................................... 35
Configuring Amplifier Support in WInWCP...................................................................................... 37
Channel Calibration Table................................................................................................................... 40
CED 1902 Amplifier ............................................................................................................................. 41
Tecella Patch Clamp Amplifier Control Panel................................................................................... 43
EPC-9/10 Patch Clamp Amplifier ....................................................................................................... 45
Molecular Devices Multiclamp 700A/B............................................................................................... 47
DCLAMP - Dynamic Clamp.................................................................................................................. 48
Recording Experimental Signals
Monitoring Input Signals & Patch Pipette Seal Test............................................................................. 51
Recording Signals...................................................................................................................................... 54
Recording Modes
Free Run................................................................................................................................................. 57
External Trigger .................................................................................................................................... 58
Detect Events......................................................................................................................................... 59
Stimulus Protocol ................................................................................................................................ 60
Setting the Recording Sweep Size and Duration .................................................................................. 61
Creating Stimulus Protocols
Opening the Stimulus/Recording Protocol Editor............................................................................ 62
Analog & Digital Output Channels..................................................................................................... 63
Recording Settings................................................................................................................................ 64
Adding Stimulus Waveforms to the Protocol ................................................................................... 66
Global Stimulus Variables.................................................................................................................... 72
Saving and Loading Stimulus Protocols............................................................................................ 74
1