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Table of Contents Warranty vii Liability viii Instructions for Returning Instruments ix Warnings xi Safety Precautions xi CHAPTER 1 Introduction 1-1 1.1 Features of the Probot™ Micro Fraction Collector 1-1 1.2 General Design of the Probot Micro Fraction Collector 1-2 1.3 Front View of Probot 1-4 1.4 The Manual Control Unit 1-4 1.5 Rear View of Probot 1-5 1.6 Control of the Probot Micro Fraction Collector 1-5 1.7 About this Manual 1-6 CHAPTER 2 Installation and Getting Started 2-1 2.1 Installation 2.1.1 Location of Probot in the Laboratory 2-1 2-2 2.2 2-3 Unpacking 2.3 Removing Shipping Components 2.3.1 Removing the Top Cover 2.3.2 Drive Table Safety Brackets 2-3 2-4 2-5 2.4 Installing the Probot with the UltiMate Capillary HPLC System 2.4.1 Electrical Connections 2-6 2-6 2.4.1 2.4.1 2.4.1 2.4.1 2.4.1 2.4.1 2.4.1 2.4.1 2.4.1 A B C D E F G H I 2.4.2 2.4.2 A 2.4.2 B RS-232 Connector START Connector REMOTE Connector TTL IN Connector INTERLOCK Connector RELAY Connector ANALOG IN Connector VIDEO Connector POWER Connector 2-6 2-6 2-7 2-7 2-7 2-7 2-8 2-8 2-8 Fluidic Connections for Fraction Collection 2-9 Connecting the µ-Tee for Matrix Addition Connecting the Dosage Module 2-9 2-10 2.5 Software Installation 2.5.1 System requirements 2.5.2 Installation of µCarrier 2-11 2-11 2-11 2.6 Introduction to the µCarrier™ Software 2.6.1 Key Features 2.6.2 Design of µCarrier 2.6.3 The Editors and Wizards 2-12 2-12 2-13 2-13 User’s Manual Probot D960R3 iii