Download SOLiD 4 System Library Preparation Guide

Transcript
Glossary
multiplexing
P1 Adaptor
Double-stranded oligonucleotide ligated at the 5′ end of the library
P2 Adaptor
Double-stranded oligonucleotide ligated at the 3′ end of the library
tag
templated bead
preparation
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Method to analyze multiple biological samples in a single spot using barcodes
A length of DNA to be sequenced
Process of adding library template to beads by emulsion PCR, enriching the beads to
remove beads without template, and modifying the 3′ end of the template on the beads
to prepare for bead deposition and sequencing
SOLiD™ 4 System Library Preparation Guide