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Section 3.1 Prepare a 2 × 50 bp mate-paired library
Shear the DNA
3
Shear the DNA
IMPORTANT! For accuracy, determine sample DNA concentration using a
double-stranded DNA-specific fluorescence assay. Assays recommended are the
Invitrogen Quant-iT™ PicoGreen® dsDNA Assay Kit (Invitrogen, P7589), the
Invitrogen Quant-iT™ dsDNA HS Assay Kit (Invitrogen, Q32851 or Q32854); or
the Invitrogen Quant-iT™ dsDNA BR Assay Kit (Invitrogen, Q32850 or
Q32853).
1. Choose the appropriate shearing method based on the desired insert size of the
mate-paired library (see Table 12 on page 54).
Note: These conditions are guidelines. A shearing trial prior to large-scale
shearing is recommended if additional DNA is available.
SOLiD™ 4 System Library Preparation Guide
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Chapter 3 Mate-Paired Library
Preparation
Prepare for
shearing