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Running an assay without the Assay Start Wizard 1. If the plate carrier is in the instrument, press the LOAD button or click the Unload button on the toolbar. The plate carrier will come out. Note! The software button shows Unload if the plate carrier is in the instrument and Load if it is out. 2. Load your first plate. Make sure the plate is orientated correctly with the A1 position in the left hand corner furthest from you. The plate should have a barcode on it to select the protocol to be used to count it. 3. Press the START button on the instrument. The plate carrier with the plate will go in to the instrument and measurement will begin. During a run you can click either the Pause or Stop buttons if you need to pause or stop the measurement. Pressing the STOP button on the instrument has the same effect. 4. You can follow the measurement by means of the plate map with colors indicating the intensity of the signal from each well. At the top of the window there is information about the status of the measurement. 5. When the plate has been measured, the plate carrier will come out and you can remove the plate. If more than one plate has been defined in the protocol then load the next one. 6. Press the START button on the instrument. 65