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Quantitative Data Analysis
Analyst Classic and IntelliQuan Integration Algorithms
The IntelliQuan algorithm uses one of two peak-finding parameters: Automatic IQA II, which is a
parameterless setting, or Specify Parameters MQ III. After integrating peaks using the IntelliQuan
algorithm, choose which peak-finding parameter best fits the data set. This is done in the
displayed peak integration parameters in the Peak Review pane or window.
Table 6-3 shows the parameters available with the Analyst Classic algorithm.
Table 6-3 Analyst Classic Algorithm
Parameter
Definition
Default Bunching Factor
The number of points to be averaged together and
considered as a single point for the purpose of peak-finding.
Default Number of Smooths
The number of times to smooth the chromatogram.
Default Void Volume Retention
Time
Any peaks that appear before this time are ignored.
Default Concentration Units
The concentration units used to describe the sample
concentration, for example, pg/µL.
Default Calculated Concentration The concentration units used to describe the calculated
Units
sample concentration, for example, pg/µL.
Default RT Window
The time window centered at the expected retention time for
peak-finding. For example, a 30 second retention time
window gives an additional 15 seconds before and after the
expected retention time.
Table 6-4 shows the parameters available with the MQ III algorithm, but not the IQA II algorithm.
Table 6-4 MQ III Algorithm
Parameter
Definition
Default Noise Percentage
The threshold used in peak-finding. Only peaks higher than
this specified percentage will be detected.
Default Baseline Subtraction
Window
A time window around each data point that is used to
determine the height of the baseline correction to be applied
to that point. This time window helps remove excessive
noise from the chromatogram. The baseline is defined as
the line connecting the point of minimum intensity on the left
side of a given data point to the point of minimum intensity
on the right side, within the specified window.
Default Peak-Splitting Factor
Controls whether a given peak cluster consists of multiple
adjacent peaks or one (possibly noisy) peak. If the intensity
dip is less than the value specified, then a single peak is
reported; otherwise the point with minimum intensity in the
dip splits the cluster into two separate peaks. Setting a large
factor will prevent clusters from being split into more than
one peak.
Default Void Volume Retention
Time
Any peaks that appear before this time are ignored.
Advanced User Guide
Release Date: March 2012
Analyst® 1.6.1 Software
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