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PBMC Analysis Snapshot of En6re Sample PE CD4 An6 Human PBMC Sample1 (Test 34) Revolutionizing Life Science Sample Snapshot Count % Debris (orange) 10,916 59% Residual RBC’s (yellow) 1,267 7% CD4-‐ Lymphocytes (green) 2717 15% CD4+ Lymphocytes (red) 2792 15% CD4-‐ monocytes (white) 435 2% CD4+ monocytes (blue) 145 1% CD4-‐ Residual Granulocytes (grey) 145 1% Comments • Dynamic range – 0.5 log, some error introduced due to minimal overlap, but es6mate this is less than 2-‐3% error contribu6on – Sufficient separa6on to quan6fy CD4 + lymphocytes (red) versus CD4-‐ lymphocytes • Impedance channel observa6ons – Clear direct sizing and counts of CD4+&-‐ lymphocytes, as well as the monocytes (blue) – Impedance channel reveals small residual granulocyte popula6on, despite Ficoll-‐Paque Prep – Enables amazing sensi6vity on count, down to as few as 129 events Cell Count % CD4+ Lymphocytes (green) 2,886 48% CD4-‐ Lymphocytes (red) 2,504 42% CD4-‐ Monoctyes (yellow) 476 6% CD4+ Monocytes (grey) NA <1% CD4-‐ Granulocytes (orange) 129 3% CD4+ Granulocytes (grey) NA <1%
