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CHAPTER 4. GETTING STARTED 56 Figure 4.28: Select your adapter trim list. You can use the default trim parameters or adjust them if necessary. Figure 4.29: Check the settings and save your results. 4.4.5 Output from the Prepare Overlapping Raw Data and Prepare Raw Data workflows Different outputs are generated from the "Prepare Overlapping Raw Data" and "Prepare Raw Data" workflows. Prepare Overlapping Raw Data. Performs quality control and trimming of the sequencing reads and merging of overlapping read pairs and generates five different outputs: 1. QC graphic report. The report should be checked by the user. 2. QC supplementary report. The report should be checked by the user. 3. Trimming report (the trimmed sequences are automatically used as input in the merging of paired reads step). The report should be checked by the user. 4. Merged reads output. Use as input together with the "Not merged reads output" in the next ready-to-use workflow (e.g. "Identify Variants WES").