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The Analysis module 13.6.1 Overview of molecular size determination How to create a molecular size curve The table below is a brief description of how to create a molecular size curve. Step Action 1 Perform a run with one or more standards to create a standard curve. 13 Note: The standards should contain a number of components of known molecular size and these should extend beyond the size limits that are expected in the test sample. 2 Peak integrate the standard curve to produce a peak table. 3 Use the peak table from the standard to produce a molecular size table. Each peak is represented by a retention value. 4 Select the relevant peaks and input data for the corresponding molecular sizes. Result: The software plots these values as a molecular size curve. This curve has an inverse relationship between the logarithm of the molecular size and retention. • p 449
