Download PowerPlex® Y23 System

PowerPlex® Y23 System
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1. Description......................................................................................................................................... 2
2. Product Components and Storage Conditions ........................................................................................ 4
3. Before You Begin................................................................................................................................. 5
3.A. Precautions ................................................................................................................................ 5
3.B. Spectral Calibration .................................................................................................................... 5
4. Protocols for DNA Amplification Using the PowerPlex® Y23 System ........................................................ 6
4.A. Amplification of Extracted DNA ................................................................................................... 6
4.B. Direct Amplification of DNA from Storage Card Punches ............................................................... 9
4.C. Direct Amplification of DNA from Swabs .................................................................................... 12
5. Instrument Setup and Sample Preparation .......................................................................................... 15
5.A. Detection of Amplified Fragments Using the Applied Biosystems® 3500 or
3500xL Genetic Analyzer with 3500 Data Collection Software, Version 1.0 ................................... 15
5.B. Detection of Amplified Fragments Using POP-4® Polymer and the ABI PRISM® 3100 or
3100-Avant Genetic Analyzer with Data Collection Software, Version 2.0, or the Applied
Biosystems® 3130 or 3130xl Genetic Analyzer with Data Collection Software, Version 3.0 ............. 26
6. Data Analysis .................................................................................................................................... 28
6.A. Importing PowerPlex® Y23 Panels, Bins and Stutter Text Files into
GeneMapper® ID-X Software, Version 1.2 .................................................................................. 28
6.B. Importing the CC5 ILS 500 IDX Size Standard into GeneMapper® ID-X Software, Version 1.2 ......... 29
6.C. Creating a Size Standard with GeneMapper® ID-X Software, Version 1.2 ...................................... 30
6.D. Creating a Casework Analysis Method with GeneMapper® ID-X Software, Version 1.2................... 31
6.E. Creating a Databasing or Paternity Analysis Method Using a Global Filter
with GeneMapper® ID-X Software, Version 1.2........................................................................... 34
6.F. Importing PowerPlex® Y23 Panels and Bins Text Files with
GeneMapper® ID Software, Version 3.2 ..................................................................................... 37
6.G. Importing the CC5 ILS 500 Size Standard into GeneMapper® ID Software, Version 3.2................... 38
6.H. Creating a Size Standard with GeneMapper® ID Software, Version 3.2 ......................................... 38
6.I. Creating a Casework Analysis Method with GeneMapper® ID Software, Version 3.2 ...................... 39
6.J. Creating a Databasing or Paternity Analysis Method Using a Global Filter
with GeneMapper® ID Software, Version 3.2 .............................................................................. 42
6.L. Results..................................................................................................................................... 45
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TMD035 · Revised 3/15
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