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OPERATION PRINCIPLES
SAMPLE ANALYSIS OVERVIEW
CBC Mode
After aspiration in the CBC mode, aliquots of the whole-blood sample are distributed as
follows (Figure 2.5):
r
The 3µL sample aliquot at the tip of the probe is discarded into the Rinse bath as the
exterior of the sample probe is rinsed, ensuring sample integrity.
r
10µL of sample is delivered to the First Dilution/Hgb bath for use in preparing the
primary RBC/Plt dilution and for measuring the Hgb value.
r
10µL of sample is delivered to the WBC/BASO bath for the WBC/BASO count.
r
7µL of remaining sample is discarded into the Rinse bath.
CBC/DIFF Mode
After aspiration in the CBC/DIFF mode, aliquots of the whole-blood sample are distributed as
follows (Figure 2.6):
r
The 3µL sample aliquot at the tip of the probe is discarded into the Rinse bath as the
exterior of the sample probe is rinsed, ensuring sample integrity.
r
10µL of sample is delivered to the First Dilution/Hgb bath for use in preparing the
primary RBC/Plt dilution and for measuring the Hgb value.
r
10µL of sample is delivered to the WBC/BASO bath for the WBC/BASO count.
r
25µL of sample is delivered to the DIFF bath for development of the DiffPlot.
r
5µL of remaining sample is discarded into the Rinse bath.
Delivery
In the CBC and the CBC/DIFF modes, each aliquotted sample is delivered to its appropriate
bath using a tangential flow (Figure 2.8) of reagent, which mixes the diluted sample and
minimizes viscosity problems.
Figure 2.8 Sample Delivery Using Tangential Flow
Probe
Reagent
input
Tangential flow
Mixing bath
PN 4237615B
7616002A
2-7
2