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Defining Assays
Editing Assay Groups
4.
Drag and drop to add assays to the new plex.
As assays are added to a plex, the Selected Plex box provides information about
the termination mixes and the minimum peak separation between any assay
analyte and any other expected assay mass peak in the multiplex. A multiplex is
invalid if there is more than one common terminator mix for the assays or the
minimum separation is too small relative to resolving the mass peaks for iPLEX
or MassEXTEND spectra. In this case, an exclamation point ("!") appears next to
the display, indicating the multiplexing of the assays may be invalid.
5.
To remove an assay from a plex, select it in the navigation tree, and then click
Remove Assay.
6.
To remove a single plex, select it in the navigation tree, and then click Remove
Plex.
7.
To remove all the plexes from the currently selected assay group, click Empty.
8.
Type the name of the assay group in the Edit Assay Group ID box, or select an
assay project from the to Assay Project drop-down list.
The assay group will be saved to the selected assay project.
9.
To check for multiple terminator mixes and minimum peak separation, check the
Validate Plexes Before Saving option.
If any plex is found to be invalid, the Save process is canceled.
Note: Selecting this option may cause the save process to take more time. Do not
check this option if you want to save time or if you want to save the assay group
as it is currently defined.
10. Click SAVE.
The assay group is saved to the selected assay project with the given ID. If an
assay group with this name already exists in the selected assay project, a message
box appears prompting you to either overwrite the existing assay group or not.
Plexes with Numeric Names
When you drag and drop assay groups into the Edit Group tab, duplicate numeric plex
names are appended with a number to indicate which duplicate it is. For example, a
plex named "5" with two duplicates would appear as "5," "5(1)," and "5(2)" in the Edit
Group tab to distinguish the three different plexes.
You can automatically reassign numeric plex names to remove duplicate names.
To reassign numeric plex names
•
On the Edit Group tab, select the Auto re-assign numeric plex IDs option so a
check mark appears in the box.
All duplicate numeric plex names are reassigned. Duplicate naming is removed,
and duplicate plex names are assigned new numeric names.
Doc 11546, R03 CO 060094
June 30, 2006
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MassARRAY® Typer 3.4 Software
User’s Guide for iPLEX™ and hME