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Chapter 3
Defining Plates
Introduction
The PlateEditor module is used to define samples and plates and to apply samples and
assays to a plate's wells.
This chapter explains how to use the PlateEditor by providing an overview of what
you will see followed by sections on how to perform specific tasks, such as: select a
plate, create a plate, define samples, apply assays and samples to wells, create an assay
group, and edit plates.
Physical Plate
A Plate in the MassARRAY Server database (database) represents the physical
microtiter plate that is spotted onto the SpectroCHIP. Since the Plate layout (96 wells
or 384 wells) also mirrors the SpectroCHIP layout, in a sense the definition of the Plate
also serves as a definition for the SpectroCHIP. Each well contains a single Sample
(which can be a pooled sample) and one or more Assays that are run simultaneously on
that Sample. Assays which are run simultaneously are called multiplexed assays.
Plate Database Hierarchy
The Plate exists within a hierarchy in the database. As defined in the database
hierarchy, a Customer has Projects, and a Project has Plates associated with it. A Plate
must exist within a single Project. The Plate hierarchy can be used to associated
Plates in any manner that is useful to you; that is, the hierarchies do not need to
actually be Customers and Projects, but can reflect some other organization of similar
form (e.g. Project and Subproject).
PlateEditor
Basics
Opening the PlateEditor
To open the PlateEditor
1.
From the MassARRAY Typer window, click PlateEditor.
Click to open the PlateEditor
The Connect to Database dialog box opens.
2.
Enter the appropriate information, and then click OK.
Once connected, the PlateEditor opens.
3.
Select the plate you want to work on, and then assign assays and samples.
See “Applying Assays and Samples to Wells” on page 46 for instructions.
Closing the PlateEditor
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Doc 11546, R03 CO 060094
June 30, 2006
On the File menu, select Exit.
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MassARRAY® Typer 3.4 Software
User’s Guide for iPLEX™ and hME