Download iPLEX Software Guide.book

Defining Assays
SNPs
Removing SNPs from SNP Groups
To remove a SNP from a SNP group
1.
In the Members box, select a SNP_ID.
The Add SNP to Group button changes to the Remove SNP from Group button.
2.
Click Remove SNP from Group.
3.
To remove all SNPs from the Members box, click Empty.
4.
Click SAVE to save your changes.
You may not save an empty SNP group to the database. Add SNPs to the SNP group if
it is currently empty. Then, click SAVE.
SNPs
Creating New SNPs
New SNPs may be created by updating existing SNPs or "from scratch."
To create a new SNP by modifying an existing SNP
1.
Select an existing SNP.
(See “Selecting SNPs” on page 20 for instructions.)
2.
In the SUSID drop-down list, select <New>.
3.
In the SNP_ID box, type a new SNP_ID.
Or, leave the SNP_ID as it appears for the selected SNP. Then, modify the
sequence in the Sequence box.
4.
Click Test to test the DNA sequence for sequence, (maximum) strand length, and
the number of SNPs to be reported.
If any errors are found, a message box appears. Make any necessary corrections to
the sequence.
5.
Click Add SNP to Group to add the SNP to the SNP group currently being
edited.
The new SNP cannot be saved to the database until it belongs to a SNP group.
The SNP_ID is added to the Members box, and it is assigned a temporary value
in the SUSID box. (The SUSID will be updated once the SNP is saved to the
database.)
Note: Once a SNP has been added to a SNP group, the SNP may no longer be edited.
Instead, you must make a copy of it; the copied SNP may be edited. Also, if any
assays were associated with the original SNP, they must be re-associated with
another SNP before the original SNP can be deleted.
6.
Click SAVE to save your changes.
To create a new SNP
1.
2.
Type the ID for the new SNP in the SNP_ID box.
Click Locate.
If the SNP_ID does not yet exist in the database, the SUSID box displays <New>,
indicating a new SNP is being created.
MassARRAY® Typer 3.4 Software
User’s Guide for iPLEX™ and hME
22
Doc 11546, R03 CO 060094
June 30, 2006