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lost, and the resulting analysis tree would be identical to the one in B08. In this example, since all of the analyses in C08 are group analyses, they are identical to the analyses in B08 – so, in this instance Replace and Retain are essentially the same. Choose Retain. The Workspace will now reflect the newly-merged trees (below). This is how you can easily add any modifications to an analysis tree from one sample to another – simply drag the whole tree! You can then choose to keep (the Retain option) or throw away (the Replace option) any differences between the common parts of the two trees. You may have noticed that the Retain or Replace dialog window did not appear when you dragged analyses to a group, even when they already existed (for example, when you were modifying the Naive, M1, M2, and M3 subsets for the CD8 analyses at the beginning of this lesson). FlowJo assumes that when you drag an analysis to a group, you want to replace all of the existing analyses that have the same name. Then, every sample that has the group’s version of those analyses will also get the new version that you have copied to the group. Dragging an analysis tree onto a group is essentially the equivalent of dragging the tree to every sample in the group, and choosing Replace. Lesson 5: Modifying Group Analyses 49