Download FlowJo - CMIMA
Transcript
What is a template? When you define a table, you will add rows to a table template. Each row defines one statistic that you want to export, such as frequency, mean fluorescence of FITC, etc. When you create the table, FlowJo cycles through each sample in the current group and requests the particular statistic defined in the table. Then, when you create the table and import it into another program, you will have one row in the table for every sample in the current group, and one column for every statistic in the table template. You may define as many table templates as you wish; you could use one for each different set of statistics. This one, as you may have guessed, will be used for generating a table of the major PBMC subsets analyzed in the first stain combination. Note that you can apply a template to any group; it doesn’t matter which group was active at the time that you defined the table template. However, most likely your table will only be applicable to one group of samples. Go back to your Workspace, and select the PBMC subsets group. From the first sample only, select the “Lymphocytes” and “CD45+” subpopulations, and the two frequency statistics under the “CD45+” subset. (shift-click to highlight the four rows.) Drag the highlighted rows and drop them into the right portion of the Table Editor window. The Table Editor will now look like it does here: Just as you can use the option drag action on analysis trees between samples, you can also use option -drag for transferring an entire tree to the Table Editor. Lesson 6: Tables and Layouts – Collating Data Output 51