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- Washer H2O tube ③: Remove and discard the old tubing. It is possible that a small quantity of distilled water will flow out of the water reservoir. Immediately dry it after the tube replacement. Carefully insert the new tubing into the pinch-valve and attach the ends to the appropriate fittings of the water reservoir and the washer. - Normally the reading cuvettes should be substituted only if it is impossible to clean them properly or of they are damaged. After substitution with new cuvette/s it is important to perform the FCC procedure for the recalculation of the correction factor of the optical path. If the FCC procedure is not performed then it may result in an optical transmission error of approximately ±3% max. - After removing cuvette plate and then replacing cuvette/s on the cuvette plate, give it a final inspection to make certain everything concerning cuvettes cleanliness and integrity is satisfactory. Gently place the cuvette plate downwards onto the rotor. Be sure it is uniformly seated and secure all the cuvettes by gently pushing downwards until they bottom against the depth of the rotor. Do not force the any cuvette into place in the cuvette plate, use a light pressure with thumb. Fasten the cuvette plate securely by tightening all the mounting screws. To ensure that pressure is applied uniformly, first tighten all mounting screws finger-tight and then tighten them in a crosswise pattern. Do not use excessive force on the screws. Note: The cleaning of cuvettes is of special importance and demands the complete removal of all contaminations from the inner surfaces. If the cuvettes are not regularly cleaned, then during the tests the slow release of contamination deposits from the inner surfaces may cause serious problems of contaminations and carry-over. In such situations it is highly recommended to perform thorough washing of cuvettes using appropriate solution during extra wash followed by a washing cycle. Consult factory for additional information regarding the washing procedure. b) ISE Module (see Figure 2, "ISE MODULE") - To access the ISE Module tubing, remove the plastic lodging for the reagents. Slide up the ISE electrodes panel to have an easy access to disconnect the waste tube connecting peristaltic pump to the ISE Module (refer to chapter L, par.1.4). - Replace the Y-shaped tubing set ⑦ by first inserting the single tube end into the bubble sensor, and then connect it to the funnel located above. Insert the remaining two tube attachments into the appropriate slots (K+ tube in the upper slot and the REF tube in the lower slot) of the pinch valve and then correctly connect their ends to the REF and K electrodes as shown in the figure. Verify correct tubing placement in the pinch valves. - Replace the ISE peristaltic pump tube ⑥ by connecting one end to the ground electrode, then around the pump head, and the other end to the waste liquid connector as illustrated. - Connect tube ⑤ to the Co2 electrode (or By-pass) and the ground electrode. - Replace peristaltic pump cartridge ④ as explained in the preceding "a) Reading Station". - Replace tube ①A connecting the peristaltic pump through pinch-valve to the dilutor. Section I Chapter N Maintenance & Troubleshooting BT3000 PLUS Rev.0, Soft Ver. 8 Page 4 of 16