Download Agilent Feature Extraction 12.0 Reference Guide

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Default Protocol Settings
GE1_1200_Jun14
Table 4
Default settings for GE1_1200_Jun14 protocol (continued)
Protocol step
Parameter
Default Setting/Value (v12.0)
Spot Deviation Limit
Hidden if Array Format is set to
Automatically Determine.
8.0 for all formats except for third
party, for which it is set to 1.5
Calculation of Spot Statistics
Method
Hidden if Array Format is set to
Automatically Determine.
Use Cookie (All Formats)
Cookie Percentage
Hidden if Array Format is set to
Automatically Determine.
0.650 (Single Density, 25k)
0.561 (Double Density, 95k)
0.700 (185k, 185k 10 uM, 244k 10
uM, 65-micron feature size)
0.750 (30-micron feature size)
Exclusion Zone Percentage
Hidden if Array Format is set to
Automatically Determine.
1.200 (All Formats except 30-micron
feature size)
1.300 (30-micron feature size)
Auto Estimate the Local Radius
Hidden if Array Format is set to
Automatically Determine.
True (Single Density, Double
Density, 25k, 95k)
False (185k, 185k 10uM, 65-micron
feature size, 30-micron feature size,
244k 10uM)
LocalBGRadius
Hidden if Array Format is set to
Automatically Determine.
100 (when False for 185k, 185k
10uM, 65-micron feature size, 244k
10 uM)
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Feature Extraction Reference Guide