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General Introductory Remarks
12
Working with Fluorescence (Option)
A supplementary fluorescence unit is available as
an option. According to the configuration this is
suitable for the following applications:
- Configuration "Basic": non-simultaneous application of fluorescence and laser functions.
- Configuration "Advanced": a simultaneous
application of fluorescence and laser functions
is possible.
For more information about the application and the
basic settings refer to the User Manual.
12.1
General Introductory Remarks
The procedure to use for working with fluorescence illumination depends on the way your system is configured. The differences concern the
manipulation of the microscope (motorized or
manual version) and the fluorescence shutter or
filter wheel respectively.
If your system is equipped with a motorized microscope, you can control all the elements that you
need to adjust specifically in order to use the fluorescence illumination via PALM RoboSoftware.
When using a manual microscope you have to
make the settings on the microscope manually;
the functions of the filter wheel and the shutter are
controlled by the PALM RoboSoftware; In other
words, you can select the desired excitation filter
or close and open the shutter via a program command.
12.2
Settings for Working with Fluorescence - Manual Microscope
Note!
Close the shutter (chapter 12.6) before
switching on the fluorescence lamp and
before finishing or interrupting your work.
This will prevent unintentional exposure of
your sample to fluorescence light.
12.3
Settings for Working with Fluorescence - Motorized Microscope
In order to use the fluorescence illumination, you
must
- switch on the fluorescence illumination (refer to
User Manual)
- select the desired fluorescence filter unit (chapter 12.4 "Activating a Fluorescence Settings
Record"). With this the functions "(Excitation)filter", "Reflector position", "Microscope
light", "Timer" and "Shutter" are performed via
a single command.
The basic settings of these functions have been
made during installation of your PALM system.
The PALM RoboSoftware provides you with the
capability of saving various combinations of
these parameters and retrieving them when
they are needed (chapter 12.9).
Note!
Close the shutter (chapter 12.6) before switching on the fluorescence lamp and before finishing or interrupting your work. This will prevent
unintentional exposure of your sample to fluorescence light.
In order to use the fluorescence illumination, you
must
- switch on the fluorescence illumination (refer to
User Manual)
- select the desired fluorescence excitation filter
(necessary when using a filter wheel, only;
refer to chapter 12.8.3)
- swivel in the desired reflector position in the
microscope (refer to User Manual)
- switch the microscope light on or off as necessary
- activate the timer (if you want to preset a time
for which the excitation light should illuminate
your sample; refer to chapter 12.7), and
- open the shutter (chapter 12.6).
PALM RoboSoftware 3.1-0106 (EN)
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