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Notes 190 • The comparative method used in regressions was BD FACSCalibur with BD Multiset™ software and BD Multitest™ IMK Kit with BD Trucount tubes. • The least-squares method was used to fit the linear regression for all subsets. • Accuracy studies were conducted over a period of 12 days at two sites. • Number of replicate determinations used to calculate each mean for X: two replicates for CD3, one for all other subsets. • Number of replicate determinations used to calculate each mean for Y: minimum of two and maximum of four replicates for CD3 and minimum of one and maximum of two for all other subsets. BD FACSCanto Flow Cytometer Reference Manual