Download Maximizer Manual - Biovest Hollow Fiber

Troubleshooting
Contamination
Sources of contamination: Termination of a culture run due to contamination is
always a humbling experience. It is important to recognize the most likely
source(s) of such a problem because it (they) must be avoided in future runs.
Sometimes diagnosing the cause of contamination is simplified by observing
whether the problem is restricted to the ICS or ECS loop. Refer to any records of
autoclave cycles, validation of hoods and autoclaves, sterility tests, medium and
serum lots, quality control procedures and any timetable that shows when
sampling or bottle replacement is performed. Be honest about the history of
aseptic techniques used throughout the run.
Cultureware and instrument cleanup: Remove the flowpath as described on
page 6-90. Save the probe cables and gassing lines, and thoroughly clean the pH
and oxygen probe case assemblies. Replace the oxygen electrode membrane, and
add fresh electrolyte. The components of the probe holders can be soaked in
sodium hydroxide solution (0.1N) for 30 minutes to remove endotoxin residue,
then rinsed thoroughly in distilled water before they are washed with tissue
culture detergent.
Properly dispose of the contents of all bottles that were attached to the flowpath.
Bottles and cap assemblies may be treated like the probe holders with sodium
hydroxide solution. Vent filters for cap assemblies should be discarded as a
biohazard.
Disinfect the incubator, the outer surfaces of the MAXIMIZER cabinet and the
interior of the door. Avoid spilling any solutions on the circulation pump rotor, in
the bulkhead connections and in the incubator exhaust vent.
Biovest International, Inc.
Operations Manual for the ACUSYST-MAXIMIZER
P/N 700037-000 Rev. H
Page 7-25