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Precautions
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AlphaScreen™ beads are light-sensitive. All assays using
the AlphaScreen beads should be performed under subdued laboratory lighting of less than 100 lux. Alternatively,
green filters (Roscolux filters #389 from Rosco, or equivalent) can be applied to light fixtures. Any incubation of
AlphaScreen™ beads should be performed in the dark.
Plates can be covered by an opaque microplate to minimize the effect of light.
•
Due to the small volumes used in the assay, it is recommended that the plates be covered with TopSeal-ATM adhesive sealing film to reduce evaporation during incubation
periods (PerkinElmer® Inc., Cat. No. 6005185). The assay
can be read with the TopSeal-A film in place.
•
The PhosphoSensor Acceptor beads contained in this kit
may slightly aggregate with time. This is normal. It is advised to vortex the beads prior to use.
•
Beads should be stored in the dark at 4oC.
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