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Table of Contents
Instrumedics Frozen Section Process Overview ……………
7
Freezing Process …………………………………………………………. 7
CryoJane Process ………………………………………………………… 7
CryoJane System Components ……………………………………..
8
CryoJane ECU …………………………………………………………….
9
CryoJane Mech/Flash Unit Components ……………………….
10
CryoJane Operating Procedure ……………………………………
11
Background ………………………………………………………………. 11
Preparation ……………………………………………………………….. 11
Use ……………………………………………………………………….. 12
Steps for a Single Section ………………………………………… 12
Steps for Multiple Sections ………………………………………. 18
Summary of the Instrumedics Frozen Sectioning Process .
19
Special Fixation … … … … … … … … … … … … … … … … … … … … … …
20
Aqueous Fixation …… ………………………………………………….. 20
“Anhydrous” Fixation …………………………………………………….. 21
CryoJane Maintenance ………………………………………………..
22
ECU ……………………………………………………………………… 22
Mech/Flash Unit………………………………………………………
5
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