Download Metabolomics Workflow Guide

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Advanced operations
Quality Control
Figure 103 Input Parameters, Filter on Sample Variability (Step 2 of 4)
d Review your filter results on the Output Views of Filter on Sample Variability
page (Filter on Sample Variability (Step 3 of 4)):
1. Review the entities that passed the filter conditions using the spreadsheet
view or the profile plot view. The total number of entities and number of entities passing the filter, along with their filtering condition, are displayed.
2. Click Back, make changes to the parameters, and click Next to return to this
view.
3. Click Next.
Figure 104 Output Views of Filter on Sample Variability, Filter on Sample Variability
(Step 3 of 4)
e Enter parameters on the Save Entity List page (Filter on Sample Variability (Step 4
of 4)):
1. Follow the steps presented in “The Save Entity List page” on page 124.
2. Click Finish.
Filter by Flags
The main goal of Filter by Flags is to remove “one-hit wonders” from further consideration. A one-hit wonder is an entity that appears in only one sample, is absent
from the replicate samples, and does not provide any utility for statistical analysis.
The new entity list is placed in the Analysis folder within the Experiment Navigator.
a Click Filter by Flags in the Workflow Browser. This operation is illustrated with
data from the “Two-variable experiment”.
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