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IVIS Spectrum
®
In vivo and in vitro optical imaging.
USERS MANUAL
March 2007
is now
a part of
IVIS® Spectrum
Hardware Manual
March 2007
© 2007 Xenogen Corporation. All rights reserved.
PN121450
Caliper LIfe Sciences
68 Elm Street
Hopkinton, MA 01748
Main Phone: 1.508.435.9500
Fax: 1.508.435.3439
www.caliperls.com
Xenogen Corporation
860 Atlantic Avenue
Alameda, California 94501, USA
Main Phone: 1.510.291.6100
Fax: 1.510.291.6196
www.xenogen.com
IVIS Imaging Systems Technology Support
Toll free phone: 888.810.8055
Fax: 508.435.0950
E-mail: [email protected]
Discovery in the Living Organism, IVIS Imaging System and Living Image are either registered trademarks or
trademarks of Xenogen Corporation. The names of companies and products mentioned herein may be the
trademarks of their respective owners. Apple, Macintosh and QuickTime are registered trademarks of Apple
Computer, Inc. Microsoft, PowerPoint and Windows are either registered trademarks or trademarks of Microsoft
Corporation in the United States and/or other countries. Adobe and Illustrator are either registered trademarks or
trademarks of Adobe Systems Incorporated in the United States and/or other countries.
IVIS® Spectrum Hardware Manual
Contents
1 Welcome
. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1
1.1 About the IVIS Spectrum Imaging System . . . . . . . . . . . . . . . . . . . . . . . . 1
1.2 IVIS Imaging Systems Technology Support . . . . . . . . . . . . . . . . . . . . . . . 3
2 Important Safety Instructions . . . . . . . . . . . . . . . . . . . . . . . . . . . 5
2.1 Definitions . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 5
2.2 Instrument Labels . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 6
2.3 Instructions . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 6
2.4 Laser Safety . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 7
2.5 Fluorescence Light Source Safety . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 7
2.6 Environmental Considerations for the System Components . . . . . . . . . . . . . . . 7
2.7 Power Considerations . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 8
2.8 Cleaning or Moving the System Components . . . . . . . . . . . . . . . . . . . . . . . 9
2.9 Other Equipment, Chemicals, or Supplies . . . . . . . . . . . . . . . . . . . . . . . . . 9
2.10 Servicing . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 10
3 Warnings . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 11
3.1 Electrical Safety . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 11
3.2 Eye Safety & Burn Hazard . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 11
3.3 Mechanical Safety . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 12
3.4 Chemical & Biological Safety . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 14
3.5 Panels, Covers & Modules . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 14
4 Legal Notices . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 15
4.1 Limited Warranty . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 15
4.2 Patents . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 16
4.3 Trademarks . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 16
5 Specifications . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 17
5.1 Electrical Power Requirements . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 17
5.2 CCD Camera . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 17
5.3 Optics . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 18
5.4 Fluorescence Imaging Components . . . . . . . . . . . . . . . . . . . . . . . . . . . 18
5.5 Environmental Requirements . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 18
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Contents
5.6 Weights & Dimensions . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 19
6 IVIS® Spectrum Components . . . . . . . . . . . . . . . . . . . . . . . . . . 21
6.1 CCD Camera . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 22
6.2 ThermoCube Chiller Unit . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 22
6.3 Imaging Chamber . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 23
6.4 Optical Components . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 24
6.5 Specimen Warming System . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 25
6.6 Laser/Galvanometer System . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 26
6.7 Crash Avoidance System . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 26
6.8 Base Enclosure . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 26
6.9 Acquisition Computer . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 27
6.10 Accessory Cable . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 27
6.11 High Reflectance Hemisphere . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 29
7 Operating the IVIS® Spectrum . . . . . . . . . . . . . . . . . . . . . . . . . . 31
7.1 Restarting the System . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 31
7.2 Gas Plumbing . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 33
7.3 Door Operation . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 35
7.4 Centering a Subject in the Field of View . . . . . . . . . . . . . . . . . . . . . . . . 35
7.5 Fluorescent Imaging . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 37
7.6 Imaging Basics . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 38
7.7 System Shut Down Procedure . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 38
8 Troubleshooting . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 41
8.1 Measured Temperature Does Not Equal the Demand Temperature . . . . . . . . . . . 41
8.2 Photographic Image Is Unacceptable . . . . . . . . . . . . . . . . . . . . . . . . . . 42
8.3 Luminescent Image Is Unacceptable . . . . . . . . . . . . . . . . . . . . . . . . . . 43
8.4 No Image Is Produced . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 44
8.5 Hardware Problems . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 44
8.6 Accessing the Area Under the Imaging Platform . . . . . . . . . . . . . . . . . . . . 46
9 Care & Maintenance . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 47
9.1 Cleaning the IVIS Spectrum . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 47
9.2 Cleaning the Lens Protection Window . . . . . . . . . . . . . . . . . . . . . . . . . 48
9.3 Changing the Emission Filters Used in Fluorescence Imaging . . . . . . . . . . . . . 49
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IVIS® Spectrum Hardware Manual
9.4 Moving the System . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 51
10 Servicing & Maintaining the ThermoCube Chiller Unit . . . . . . . . . . . . 53
10.1 Required Tools . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 53
10.2 Turning Off Electric Power to the Main Console . . . . . . . . . . . . . . . . . . . 53
10.3 Removing the Console Rear Panel . . . . . . . . . . . . . . . . . . . . . . . . . . . 54
10.4 Sliding the Tray Out . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 55
10.5 Filling the ThermoCube Unit . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 56
10.6 Reassembling the System . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 57
10.7 Restarting the IVIS Spectrum . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 57
Appendix A Spare Parts List . . . . . . . . . . . . . . . . . . . . . . . . . . . . 59
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Contents
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IVIS® Spectrum Hardware Manual
1 Welcome
About the IVIS Spectrum Imaging System . . . . . . . . . . . . . . . . 1
IVIS Imaging Systems Technology Support . . . . . . . . . . . . . . . 3
1.1 About the IVIS Spectrum Imaging System
The IVIS® Spectrum Imaging System is a high-sensitivity, low noise, in vivo
imaging technology platform that enables noninvasive visualization and
tracking of cellular and genetic activity within a living organism in real time
(Figure 1.1). The system provides both bioluminescence and fluorescence
imaging capability.
For fluorescence imaging, the instrument can operate in reflectance or
transillumination mode. Filtered light from a broad-band lamp provides the
excitation source in both modes. In the reflectance mode, light is delivered to
four reflectors that are located on the ceiling of the imaging chamber. In the
transillumination mode, the excitation light is delivered to an x-y translation
assembly under the stage and focused to a 2 mm diameter beam that can be
directed to a particular location on the underside of the animal subject. The
system includes ten excitation and 18 emission filters that enable spectral
scanning of reporters over the range from 480-850 nm.
3D Tomography
The system also includes a structured light projector that enables reconstruction
of the surface topography. The 3D location and concentration of fluorescent
sources can be computed from structured light and transillumination fluorescent
images. The 3D location and strength of luminescent sources is computed from
structured light and luminescent image data.
1
1. Welcome
CCD
camera
XGI-8 Gas
Anesthesia
System
High resolution monitor
Imaging
chamber
Acquisition
computer
XWS-260 Workstation
Compartment that contains
the ThermoCube chiller unit
and camera power supply
Figure 1.1 IVIS® Spectrum Imaging System
The IVIS Spectrum is an integrated imaging system that includes a:
• CCD camera mounted on a light-tight imaging chamber
• Camera power supply
• Thermoelectric cooling unit
• Fluorescence light source
• Windows®-based computer system for data acquisition and analysis.
The sound-limiting enclosure beneath the imaging chamber contains the
thermoelectric cooling unit, camera power supply, and other imaging chamber
functions. All components except the computer are integrated into a single,
moveable chassis.
This manual explains how to operate and maintain the equipment, and provides
guidelines for obtaining the best bioluminescent or fluorescent images. Before
using the IVIS Spectrum, please read this manual carefully to obtain safe,
optimum performance and a maximum service life from the unit. For
instructions on using the system software, please see the Living Image®
Software User’s Manual.
If you have questions regarding this manual or the IVIS Imaging System, please
call IVIS Imaging Systems Technology Support.
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IVIS® Spectrum Hardware Manual
1.2 IVIS Imaging Systems Technology Support
Technical Support:
Toll free (888) 810-8055
1.510.291.6275 (International)
E-mail:
[email protected]
Fax:
1.508.435.9761
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1. Welcome
[This page intentionally blank.]
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IVIS® Spectrum Hardware Manual
2 Important Safety Instructions
Definitions . . . . . . . . . . . . . . . . . . . . . . . . . . . .
Instrument Labels . . . . . . . . . . . . . . . . . . . . . . . .
Instructions . . . . . . . . . . . . . . . . . . . . . . . . . . .
Laser Safety . . . . . . . . . . . . . . . . . . . . . . . . . . .
Environmental Considerations for the System Components
Power Considerations . . . . . . . . . . . . . . . . . . . . .
Cleaning or Moving the System Components . . . . . . . .
Other Equipment, Chemicals, or Supplies . . . . . . . . . .
Servicing . . . . . . . . . . . . . . . . . . . . . . . . . . . .
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2.1 Definitions
When you see the caution, warning, or voltage symbol, pay particular attention
to the safety information presented. Observance of safety precautions will help
avoid actions that could damage or adversely affect the performance of the IVIS
Spectrum. If the equipment is used in a manner not specified in this manual, the
protection provided by the equipment may be impaired.
!
CAUTION
CAUTION! This symbol indicates a potentially hazardous situation
which, if not avoided, may result in minor or moderate injury and/or
mechanical damage. It is also used to alert against unsafe practices. It
reminds you that all safety instructions should be read and understood
before installation, operation, maintenance, or repair of this instrument.
!
WARNING
WARNING! Used when an action or condition may potentially cause serious
personal injury or loss of life. Mechanical damage may also result.
VOLTAGE
VOLTAGE! Indicates high voltage or risk of electric shock.
5
2. Important Safety Instructions
2.2 Instrument Labels
Pay careful attention to the labels on the instrument.
Label
Description
The laser caution label alerts you to procedures or situations that could potentially
expose you to laser light. This label appears on the instrument behind the front
door and above the imaging chamber to indicate there is potential for exposure to
laser light in the imaging chamber.
Laser caution label
Where located on the IVIS system, the read manual label indicates that important
information about the labeled area can be found in the IVIS Spectrum Hardware
Manual.
Read manual label
Where located on the IVIS system, the pinch hazard label indicates the location of
a potential pinch point.
Pinch hazard label
2.3 Instructions
Read Instructions
Read and understand all the safety and operating instructions before you install,
operate, or perform maintenance on this product. Make sure that you fully
understand the following safety instructions, warnings, and disclaimers before
proceeding to the rest of the manual.
Retain Instructions
Retain the safety and operating instructions for future reference.
Follow Instructions
Follow all operating and handling instructions. Failure to follow operating or
handling instructions may void any warranty covering this product.
Heed Warnings
Abide by all warnings on the product and in the operating instructions. Failure
to adhere to warnings or safety precautions may void any warranty covering this
product.
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IVIS® Spectrum Hardware Manual
2.4 Laser Safety
The IVIS Spectrum is equipped with a Class II laser similar to the type used for
laser pointers (wavelength 532nm, output < 1mW). Users are protected from
eye damage due to the eye’s physiological response to bright light by blinking.
!
WARNING
WARNING! A laser beam can be harmful to the eyes. ABSOLUTELY DO
NOT look directly into the laser beam when the laser is turned on. Be aware
that the laser light can be easily reflected off of shiny surfaces and that the
reflected light can be dangerous. When the laser is on, remove all watches
and jewelry before placing hands in the imaging chamber.
2.5 Fluorescence Light Source Safety
!
WARNING
WARNING! The IVIS Spectrum is equipped with a strong bottom illumination
fluorescence light source. Do not stare directly into this source.
2.6 Environmental Considerations for the System Components
Location for the IVIS®
Spectrum
Before the IVIS® Spectrum is installed, consider the proper environment for the
components.
Install the equipment in an environment where:
• The temperature does not change or fluctuate widely. Choose an
environment where the temperature is maintained between 15-25° C (5978° F).
• The humidity does not exceed 80%.
• No strong electric or magnetic fields exist.
• No vibrations are present.
• No corrosive gases are present.
• High amounts of dust are not present.
• No open flame is present.
• There is sufficient space behind the IVIS Spectrum equipment. A
minimum space of six inches from the flat surface of the rear panel should
be provided behind the IVIS Spectrum to provide unobstructed air flow
and access to the main power on/off switch.
• The floor is level and structurally sound.
Heat
The system should be situated away from heat sources such as open flames,
radiators, heat registers, stoves, and other heat-generating electrical equipment.
7
2. Important Safety Instructions
Water & Moisture
VOLTAGE
VOLTAGE! Do not use this product near water (for example, near a sink or
wet room) due to risk of electric shock, electrical damage, and/or failure.
Laboratory Space &
Ventilation Requirements
The IVIS® Spectrum requires a minimum of 100 square feet (9 square meters)
of laboratory space for adequate ventilation. At least of six inches of space is
required behind the rear flat panel to provide unobstructed air flow.
Allocate an area at least 36 inches wide and 51 inches deep for the imaging
system console to allow the door to be fully opened and provide the
recommended air space behind the instrument.
An additional area that is 48 inches wide should be provided (preferably on the
right hand side) for a table or cart to hold the computer and provide a work
surface.
2.7 Power Considerations
Power Sources
The IVIS® Spectrum is configured for the voltage requirements of the
installation locality that was specified at the time of order. If the system is
moved to another area, check to make sure that the same voltage requirements
exist.
An IVIS Spectrum that operates on 120VAC requires a dedicated 20 Amp
circuit that is not shared with other loads except the computer and the optional
XGI-8 Gas Anesthesia System. Make sure that all equipment is plugged into a
properly grounded AC supply.
The computer should share the same circuit to avoid ground loops. Never use
auxiliary power outlets on other equipment to supply the IVIS Spectrum. Since
the system contains internal surge protection, it must be powered directly from
the main electrical supply wall socket. Use the surge protector provided by
Xenogen for the computer and other accessories.
An IVIS Spectrum configured for 230 VAC requires a dedicated minimum 10
Amp circuit subject to the same conditions noted above. For more details on the
operating requirements, see Electrical Power Requirements, page 17.
VOLTAGE
VOLTAGE! The IVIS Spectrum can operate at multiple voltages (100, 120,
220, 240 VAC); however, you are not permitted to change the input voltage
to any of the system components. Several internal modifications are required
for voltage change. If the operating voltage must be changed, contact IVIS
Imaging Systems Technology Support.
Power Cord Protection
Power supply cords should be routed so that they are unlikely to be walked on
or pinched by items placed upon or against them. Pay close attention to
receptacles and to points of connection between cords and equipment.
Lightning & Power Line
Surges
The IVIS® Spectrum is supplied with an internal surge protector that is not
customer serviceable. Contact IVIS Imaging Systems Technology Support
regarding equipment servicing.
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IVIS® Spectrum Hardware Manual
A separate surge protector is provided for the computer and other accessory
equipment.
Facilities should be adequately wired according to local building codes.
Power Outages
If the IVIS Imaging System experiences a loss of supply power, turn off the
power switch for all components and do not restart the system until reliable
power has been restored. For more details on how to restart the system, see
Startup Procedure, page 32.
Overloading
!
WARNING
WARNING! Do not overload wall outlets, extension cords, or integral
convenience receptacles as this can result in a risk of fire or electric shock.
See Electrical Power Requirements, page 17 for requirements.
2.8 Cleaning or Moving the System Components
Cleaning/Liquid Entry
VOLTAGE
VOLTAGE! Do not use liquid or aerosol cleaners and never spill liquid of any
kind on any of the IVIS® Spectrum components. Sprays and liquids that come
into contact with the IVIS System may result in damage to the system or
electrocution. For more details on proper care of the system, see Cleaning the
IVIS Spectrum, page 47.
Moving the IVIS® Spectrum
The IVIS® Spectrum is a sensitive, scientific instrument and should be moved
with care. Pay particular attention when rolling the instrument on its casters to
avoid toppling the equipment.
The total height of the instrument is 83 inches (211cm). If necessary, the plastic
dome can be removed for easier passage through doorways. For more details on
removing the dome, see page 51.
If you have any questions about moving the instrument, contact IVIS Imaging
Systems Technology Support.
2.9 Other Equipment, Chemicals, or Supplies
Use of any equipment other than that recommended by this manual has not been
evaluated for safety and, therefore, is the sole responsibility of the user.
No chemicals are required for the operation of the IVIS Spectrum. Other usersupplied chemicals or materials may be required as part of your specific
biological testing procedures.
9
2. Important Safety Instructions
2.10 Servicing
Refer all servicing to IVIS Imaging Systems Technology Support. If the product
is damaged and requires service, unplug the product from the outlet and contact
technical support. Servicing by anyone other than those authorized by Xenogen
voids any warranty covering this product.
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IVIS® Spectrum Hardware Manual
3 Warnings
Electrical Safety . . . . . . . .
Eye Safety & Burn Hazard . .
Mechanical Safety . . . . . .
Chemical & Biological Safety
Panels, Covers & Modules . .
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3.1 Electrical Safety
!
WARNING
WARNING! DO NOT attempt to service the IVIS Spectrum yourself. Contact
IVIS Imaging Systems Technology Support for electrical service needs.
Although there are no voltages in excess of 45V inside the imaging chamber,
local line voltages can be present inside the electronics cabinets.
!
CAUTION
CAUTION! If cleaning is necessary, wipe the exterior surfaces of the
IVIS Spectrum with a soft, damp cloth only. Do not allow fluids of any
kind to enter the system interior under any circumstances. For more
details on cleaning the system, see page 47.
3.2 Eye Safety & Burn Hazard
!
WARNING
WARNING! The IVIS Spectrum is equipped with a Class II laser similar to the
type used for laser pointers (532nm, output < 1mW). Users are protected from
eye damage due to the eye’s physiological response to bright light by blinking.
A laser beam can be harmful to the eyes. ABSOLUTELY DO NOT look
directly into the laser beam when the laser is turned on. Be aware that laser
light can be easily reflected off of shiny surfaces and that this reflected light
can be dangerous. When the laser is on, remove all watches and jewelry
when placing hands in the imaging chamber.
The IVIS Spectrum is equipped with a fluorescence light source module and
connecting fiber optic cables that are capable of producing intense light that
could be damaging to the eyes. Additionally, the system is equipped with a
Class II laser, which could be an eye hazard.
11
3. Warnings
Figure 3.1 Laser beams for alignment grid
3.3 Mechanical Safety
The IVIS® Spectrum has many internal motorized components that can move
only when the door is closed and locked. Because the imaging platform moves
frequently during routine use, keep loose objects away from the edges of the
platform where they could become jammed.
Figure 3.2 IVIS Spectrum, imaging chamber door
!
CAUTION
CAUTION! DO NOT defeat any of the safety interlocks. Do not place
anything under the imaging platform.
The imaging platform cannot be moved unless the door is closed and locked. If
the platform moves when the door is unlocked, shut down the system and
contact IVIS Imaging Systems Technology Support.
The IVIS Spectrum contains several delicate optical and mechanical
components. Do not touch these components and avoid sprays or other
contaminates that may damage lenses or other optical-mechanical components.
12
IVIS® Spectrum Hardware Manual
!
CAUTION
CAUTION! The laser/galvanometer is a very delicate optical
component that is visible when the filter wheel side access panel is
removed (Figure 3.3). DO NOT touch this device or attempt to clean or
adjust it.
Filter wheel panel
Figure 3.3 Filter wheel removable panel
The laser beam is directed into the imaging chamber through an opening at the
top of the chamber. Never place any object, tool, or fingers into this opening,
otherwise damage to the galvanometer will result.
!
WARNING
WARNING! DO NOT look directly into this opening when the laser is
operating.
13
3. Warnings
3.4 Chemical & Biological Safety
Normal operation may involve the use of test samples that are pathogenic, toxic,
or radioactive. It is your responsibility to ensure that all necessary safety
precautions are taken before such materials are used.
Dispose of all waste materials according to appropriate environmental health
and safety guidelines.
It is your responsibility to decontaminate the IVIS® Spectrum before requesting
service by IVIS Imaging Systems Technology Support. Ask your laboratory
safety officer to advise you about the level of containment required for your
application and about the proper decontamination or sterilization procedures to
follow.
Handle all infectious samples according to good laboratory procedures and
methods to prevent the spread of disease.
3.5 Panels, Covers & Modules
The imaging chamber door and the filter wheel access panel are the only user
removable panels. There are no user serviceable components in the electronics
cabinets of the IVIS® Spectrum. Do not remove the electronics cabinet covers
from the system console unless instructed by and under the supervision of IVIS
Imaging Systems Technology Support.
14
IVIS® Spectrum Hardware Manual
4 Legal Notices
Limited Warranty . . . . . . . . . . . . . . . . . . . . . . . . . . . . .
Patents . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .
Trademarks . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .
15
16
16
4.1 Limited Warranty
Xenogen Corporation ("Xenogen") provides the following limited warranty for
each new IVIS® Imaging System ("System") purchased from Xenogen as
follows ("Limited Warranty"):
i. This Limited Warranty for the System extends for a period of one (1) year
following installation of the System. This Limited Warranty is valid only
for the original customer, purchaser, or user ("Customer") and is not
assignable or transferable to any successor.
ii. During the Limited Warranty period, Xenogen will repair or replace, at
Xenogen's sole option, any defective parts if such repair or replacement is
needed because the System fails to materially conform with the published
System Specifications at the time of sale. Repairs and replacements under
the Limited Warranty will be made at Xenogen's expense. Xenogen's
liability under the Limited Warranty shall be limited to the purchase price
of the Imaging System. Xenogen shall not be liable for any other losses or
damages.
iii. No coverage or benefits shall be provided under this Limited Warranty if
any of the following conditions apply:
a. The System has been subjected to abnormal use, abnormal conditions,
unauthorized modifications (of hardware, firmware, or software),
unauthorized repair or servicing, misuse, neglect, abuse, accident,
alteration, any use inconsistent with or in contradiction to the
instructions in this manual, or other acts which are not the fault of
Xenogen.
b. Xenogen was not advised in writing by the Customer of the alleged
defect or malfunction of the System within thirty (30) days after
discovery of the defect.
iv. If a problem develops during the Limited Warranty period, the Customer
shall contact IVIS Imaging Systems Technology Support for assistance.
v. THE FOREGOING LIMITED WARRANTY IS THE CUSTOMER'S
SOLE AND EXCLUSIVE REMEDY AND IS IN LIEU OF ALL OTHER
WARRANTIES, EXPRESS OR IMPLIED. XENOGEN SHALL NOT BE
LIABLE FOR SPECIAL, INCIDENTAL OR CONSEQUENTIAL
DAMAGES, INCLUDING BUT NOT LIMITED TO LOSS OF
ANTICIPATED BENEFITS OR PROFITS, LOSS OF SAVINGS OR
REVENUE, PUNITIVE DAMAGES, LOSS OF USE OF THE SYSTEM
OR ANY ASSOCIATED EQUIPMENT, COST OF CAPITAL, COST OF
ANY SUBSTITUTE EQUIPMENT OR FACILITIES, DOWNTIME,
THE CLAIMS OF ANY THIRD PARTIES, INCLUDING
CUSTOMERS, AND INJURY TO PROPERTY, RESULTING FROM
15
4. Legal Notices
THE PURCHASE OR USE OF THE SYSTEM OR ARISING FROM
BREACH OF THE WARRANTY, BREACH OF CONTRACT,
NEGLIGENCE, TORT, OR ANY OTHER LEGAL OR EQUITABLE
THEORY, EVEN IF XENOGEN KNEW OF THE LIKELIHOOD OF
SUCH DAMAGES. XENOGEN SHALL NOT BE LIABLE FOR
DELAY IN RENDERING SERVICE UNDER THE LIMITED
WARRANTY, OR LOSS OF USE DURING THE PERIOD THAT THE
SYSTEM IS BEING REPAIRED.
vi. Some countries, states or provinces do not allow the exclusion or limitation
of implied warranties or the limitation of incidental or consequential
damages for certain products or the limitation of liability for personal
injury, so the above limitations and exclusions may be limited in their
application to you. When any implied warranties are not allowed to be
excluded in their entirety, they will be limited to the duration of the
applicable written warranty. This Limited Warranty gives you specific
legal rights which may vary depending on local law.
vii. This Limited Warranty shall be governed by the laws of the
Commonwealth of Massachusetts, U.S.A., excluding its conflicts of laws
principles and excluding the United Nations Convention on Contracts for
the International Sale of Goods.
4.2 Patents
The detection and imaging of light originating within mammals is the subject of
several issued patents and pending patent applications in the United States and
around the world, including U.S. Patent Numbers 5,6200,135, 6,217,847,
6,649,143, 6,890,515, and European Patent Commission Number EP0861093,
for which Xenogen Corporation is the exclusive licensor. The use of an IVIS®
Imaging System for such applications requires a sublicense from Xenogen
Corporation.
In addition, many of the hardware and software components of the Imaging
System are the subject of various issued patents and pending patent applications
owned by Xenogen, including: United States Patent Number 6,614,452
(Graphical User Interface for In Vivo Imaging) and 6,775,567 (Improved
Imaging Apparatus); and United States Patent Applications 09/905668 (Multiview Imaging Systems), 10/606976 (Method and Apparatus for 3-D
Reconstruction of Light Emitting Sources), 10/151463 (Method and Apparatus
for Determining Target Depth, Brightness, and Size Within a Body Region), 10/
189886 (Fluorescence illumination assembly for an imaging apparatus), and 10/
068573 (Light calibration device for use in low level light imaging systems).
4.3 Trademarks
IVIS and Living Image are registered trademarks of Xenogen Corporation. The
names of companies and products mentioned herein may be the trademarks of
their respective owners. Microsoft and Windows are either registered
trademarks or trademarks of Microsoft Corporation in the United States and/or
other countries. Pentium III is a registered trademark of Intel Corporation.
16
IVIS® Spectrum Hardware Manual
5 Specifications
Electrical Power Requirements . .
CCD Camera . . . . . . . . . . . . .
Optics . . . . . . . . . . . . . . . .
Fluorescence Imaging Components
Environmental Requirements . . .
Weights & Dimensions . . . . . . .
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18
18
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5.1 Electrical Power Requirements
Electrical Power Requirements
Specification
Voltage Required (main console)
or
Recommended Dedicated Circuit
100-120VAC 50/60Hz 15A
220-240VAC 50/60Hz 10A
20 Amps for 120VAC or
10 Amps minimum for 230VAC
For more details, see Power Considerations,
page 8.
5.2 CCD Camera
CCD Camera
Specification
Sensor Type
Back illuminated
CCD Format
2048 x 2048 pixels
CCD Size
26 x 26 mm
Effective CCD Format
1920 x 1920 pixels
Pixel Dimensions
13.5 x 13.5 μm
Quantum Efficiency
~85% 400-700nm
>50% 350-900nm
Readout Noise
<5 electrons RMS
Dark Current
<100 electrons/s/cm2 (-90° C)
Minimum Detectable
Luminance
<70 photons/s/sr/cm2
CCD Temperature
Nominal -90° C
17
5. Specifications
5.3 Optics
Optical Component
Specification
Lens f/stop
f/1- f/8
Field of View (FOV)
3.9, 6.5, 13, 19.5, and 26 cm
Resolution
>60 μm (FOV = 3.9, f/1)
Scanning Laser
<1 mW at 532 nm
5.4 Fluorescence Imaging Components
Fluorescence Imaging
Component
Specification
Excitation Filters
12 positions, 25 mm diameter, 10 filters supplied
Emission Filters
22 positions, 60 mm diameter, 18 filters supplied
Background
(Autofluorescence and
Leakage)
1 part in 107 typical
Lamp
150 W quartz halogen
21 V
3250° Kelvin
5.5 Environmental Requirements
Environmental Component Specification
18
Temperature
15 -25° C (59-78° F)
Humidity
0-80% non-condensing
Type of Use
Indoor
Sound Level
61 dB < 500 Hz
Stage Temperature
Ambient to 40° C
Altitude Rating
<2000 meters (6560 ft.)
IVIS® Spectrum Hardware Manual
5.6 Weights & Dimensions
Item
Specification
Weight
275 kg (600 lbs)
Depth
77 cm (30 inches)
Width
65 cm (25.5 inches)
Height
211 cm (83 inches)
Imaging Chamber Internal
Dimensions
51 x 51 x 66 cm (D x W x H)
19
5. Specifications
[This page intentionally blank.]
20
IVIS® Spectrum Hardware Manual
6 IVIS
®
Spectrum Components
CCD Camera . . . . . . . . . .
ThermoCube Chiller Unit . . .
Imaging Chamber . . . . . . .
Optical Components . . . . .
Specimen Warming System .
Laser/Galvanometer System .
Crash Avoidance System . . .
Base Enclosure . . . . . . . .
Acquisition Computer . . . .
High Reflectance Hemisphere
Accessory Cable . . . . . . .
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22
22
23
24
25
26
26
26
27
29
27
The IVIS® Spectrum includes the following components:
• Scientific imaging charged coupled device (CCD) camera.
• Imaging chamber with specimen warming system.
• Thermoelectric camera cooling system.
• System of lenses and filters for optimum image acquisition.
• Laser/galvanometer.
• Photographic illumination system.
• Integrated fluorescence system.
• Imaging platform crash avoidance system.
These components are integrated into one freestanding instrument that is
equipped with casters (Figure 6.1).
A pre-configured Windows®-based computer enables you to control the system
as well as acquire and analyze images.
!
IMPORTANT
ALERT! If you modify the IVIS Imaging System in any way, without prior
approval from Xenogen, all warranties that cover this product are void. In
addition, the computer included with the IVIS Spectrum is specifically
configured to run all system-related applications. Any modification of existing
software or hardware voids all warranties.
If you have any questions, please contact IVIS Imaging Systems Technology
Support.
21
6. IVIS® Spectrum Components
CCD camera
Integrated fluorescent
imaging features:
Custom lens, 11-position
filter wheel assembly,
laser light alignment
Scanning laser
assembly
Emission filter wheel
Excitation filter wheel
Imaging chamber
Gas anesthesia
manifold
Heated platform
Top illumination
light source
Bottom illumination
light source
Compartment that
contains the ThermoCube
chiller unit and camera
power supply
Figure 6.1 IVIS Spectrum components
The system is housed in a portable cart with 25 x 34” footprint.
6.1 CCD Camera
The camera is a scientific grade, thermoelectrically-cooled, back-thinned, backilluminated, large format CCD manufactured by Spectral Instruments (Figure
6.1). The camera uses a ThermoCube water chiller. Two flexible plastic water
lines connect the CCD camera to the ThermoCube chiller unit.
The CCD camera has a heated, vacuum sealing window to prevent condensation
that can obscure the image or damage other components.
The camera power supply is housed in the base of the instrument and maintains
the camera operating temperature when the computer is turned off. It has a 16bit digitized, low noise electronic readout for extremely low background
images.
6.2 ThermoCube Chiller Unit
The ThermoCube unit is a thermoelectric water chiller (Figure 6.2). Water from
the thermoelectrically cooled CCD camera is pumped to the ThermoCube unit
where the water is cooled and returned to the camera.
22
IVIS® Spectrum Hardware Manual
Figure 6.2 ThermoCube chiller unit
6.3 Imaging Chamber
The imaging chamber (Figure 6.3) is a highly specialized device consisting of the
imaging chamber housing, a heated, moveable platform, an auto focusing lens
system with f/stop control, and synchronized filter wheels that control the
spectral content of the luminescent or fluorescent images.
The LED-based illumination system and a laser-based galvanometer provide
the means to acquire photographic information and structured light before
bioluminescent or fluorescent imaging. The imaging chamber is equipped with
gas anesthesia inlet and outlet ports for use with the optional Xenogen XGI-8
Gas Anesthesia System.
The imaging chamber is light tight, so that no light penetrates from the outside
after the magnetically activated door is locked. The magnetic latch ensures the
door cannot be inadvertently opened during an imaging session.
The interior of the imaging chamber is constructed from materials that are nonphosphorescent and non-fluorescent to prevent internal light contamination that
could compromise sample measurements.
!
WARNING
WARNING! Under no circumstances should you attempt to make any
mechanical modifications to the imaging chamber.
23
6. IVIS® Spectrum Components
Door locking hardware
Figure 6.3 IVIS® Spectrum, imaging chamber
6.4 Optical Components
Imaging System/Lenses
The IVIS® Spectrum is equipped with a rotating lens carousel that provides
demagnification of 1.5X, 2.5X, 5X, and 8.8X power (Figure 6.4).The filter wheel
settings are selected in the Living Image® software. For more details, see the
Living Image Software Manual.
Power
8.8X
FOV
22.5 cm
Position D
5.0X
13 cm
C
2.5X
6.5 cm
B
1.5X
3.9 cm
A
Figure 6.4 Example field of view obtained with different lens configurations
The automatic shutter and f/stop iris is integrated into the lens system. A
window at the top of the imaging chamber protects the lenses and other optical
components in the lens compartment from contamination. For more details on
the care of this window, see Cleaning the Lens Protection Window, page 48.
NOTICE
Do not touch the glass window or permit specimens to come in contact with
the window, otherwise image quality may be impaired. If the window is struck
by a hard object, it may crack or shatter.
24
IVIS® Spectrum Hardware Manual
Emission & Excitation
Filter Wheels
There are two 11-position filter wheels. Filter settings are selected by software
control. For more details on how to access the filter wheels and change filters,
see page 49.
Figure 6.5 Filter wheel
For fluorescence studies, the IVIS® Spectrum Imaging System can illuminate a
specimen from the top of the imaging chamber or from the bottom of the stage.
A 12-position excitation filter wheel module is located in the back of the
instrument (not accessible to users). If the filters must be changed or the module
requires service, contact IVIS Imaging Systems Technology Support.
For more details on fluorescence imaging, see page 37.
6.5 Specimen Warming System
The temperature-controlled imaging platform keeps specimens warm during
imaging. The temperature is controlled by the software, and is only enabled
when the instrument is initialized from Living Image® software. The default
temperature upon initializing the instrument is 37° C.
The imaging platform does not have active cooling. Allowing the platform to
cool from 37° C to ambient temperature may require up to 20 minutes.
Temperature controlled imaging platform
Figure 6.6 Imaging chamber
25
6. IVIS® Spectrum Components
6.6 Laser/Galvanometer System
The laser/galvanometer system comprises a Class II laser (532 nm, output <
1mW). The laser beam is directed into the imaging chamber by two computercontrolled mirrors.
!
WARNING
WARNING! A laser beam can be harmful to the eyes. ABSOLUTELY DO
NOT look directly into the laser beam when the laser is turned on. Be aware
that laser light can be easily reflected off of shiny surfaces and that this
reflected light can be dangerous. When the laser is on, remove all watches
and jewelry when placing hands in the imaging chamber.
The laser/galvanometer system provides field of view alignment targets for
reproducible specimen positioning and structured light for 3D contouring and
auto-focusing.
6.7 Crash Avoidance System
The crash avoidance system uses a scanning laser to determine the subject
height and verify that it is within acceptable limits. This system prevents injury
to specimens and damage to delicate optical components such as the lens
window or the illumination diffusers for field of view A only.
You are prompted at the start of each imaging session to input a sample height
estimate. If the estimate is in error, the crash avoidance system protects the
specimen. Alternatively, the system can be configured to automatically
determine the sample height.
6.8 Base Enclosure
!
IMPORTANT
ALERT! Do not block the ventilation openings on the base or position the
system too close to a wall so that the exhaust flow is obstructed.
The base is equipped with four casters that enable you to move the instrument
(Figure 6.7). For more details on how to move the IVIS Spectrum, see page 51.
26
IVIS® Spectrum Hardware Manual
Ventilation openings
Ventilation openings
Figure 6.7 IVIS Spectrum, back view
6.9 Acquisition Computer
The system includes a Windows®-based personal computer. The Living Image®
software as well as Microsoft Office software is installed on the computer. The
Living Image software controls the IVIS Spectrum, and displays and analyzes
the image data. A 20-inch monitor and a 10/100 Ethernet network adapter card
are also included. A printer may be attached to the computer.
6.10 Accessory Cable
The IVIS Spectrum is equipped with an electrical connector that enables the
transfer of signals to or from the back panel of the instrument. The back panel
connector (Figure 6.8) is accessible at the rear of the instrument and the stage
connector is located inside the imaging chamber (Figure 6.9). Table 6.1 shows
guidelines for pin usage.
Table 6.1 Connector pin usage
Pin Number
Function
1 to 5
These pins are optimized for transport of ECG (EKG) signals and use
shielded cabling that maximizes the signal-to-noise ratio. An off-theshelf ECG monitor may be connected to this connector (for example,
the THM100, Indus Instruments). Pin 1 (ECG ground) in this particular
case, may be left unconnected.
6 to 9
Use these pins to connect a pulse-oxymetry system (for example, the
Nonin OEM III module (internal OEM Oxymetry), Nonin.com)
10 and 11
Use these pins to connect to an Infusion pump that you mount inside
the IVIS Spectrum imaging chamber.
27
6. IVIS® Spectrum Components
Table 6.1 Connector pin usage
Pin Number
Function
12 and 13
Use these pins to monitor the skin or rectal temperature of the animal.
The use of a copper-constantan thermocouple is recommended.
These pins may also be used to connect to the THM 100 ECG monitor
(Indus Instruments).
Note: A 15 pin D-sub male mating connector is required (supplied by user).
Figure 6.8 Back panel connector
Note: Connector type in shelf--MS3119E16-26PS. Mating connector required (supplied by
customer). Manufacturer: Amphenol Aerospace, part no. MS3126F16-26P.
Figure 6.9 Stage connector
28
IVIS® Spectrum Hardware Manual
6.11 High Reflectance Hemisphere
You can special order a high reflectance hemisphere that is used to check for
light contamination in the imaging chamber (Figure 6.10).
The hemisphere does not emit any photons when it is imaged in a light-tight and
contaminant-free imaging chamber. As a result, it is not visible in a luminescent
image. If a light leak or an internal phosphorescent contaminant is present in the
imaging chamber, the hemisphere reflects that light and is visible in a
luminescent image. If you suspect that there is light contamination in the
imaging chamber, see Light contamination-External, page 43.
Figure 6.10 High reflectance hemisphere
29
6. IVIS® Spectrum Components
[This page intentionally blank.]
30
IVIS® Spectrum Hardware Manual
7 Operating the IVIS
®
Spectrum
Restarting the System . . . . . . . . . .
Gas Plumbing . . . . . . . . . . . . . . .
Door Operation . . . . . . . . . . . . . .
Centering a Subject in the Field of View
Fluorescent Imaging . . . . . . . . . . .
Imaging Basics . . . . . . . . . . . . . .
System Shut Down Procedure . . . . . .
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31
33
35
35
37
38
38
The IVIS Spectrum is intended for use in biophotonic or fluorescent imaging
procedures. The system is designed to detect extremely low-level light
emissions that are orders of magnitude dimmer than can be detected by the
naked eye. The IVIS Spectrum allows you to monitor and record cellular and
genetic activity within a living organism, in real time. The imaging system
captures, quantifies, and images the light emitted by a sample.
7.1 Restarting the System
NOTICE
All components of the IVIS Spectrum should be left on unless the system will
not be used for more than 30 days. It is also important to leave the system on
to enable automatic overnight electronic background measurements.
Periodically rebooting the computer is permissible and does not affect the
camera operation.
Electrical Control & Status
Lights
The IVIS Spectrum console has two electrical switches and one status light
(Figure 7.1). The color of the status light indicates the state of the instrument
(Table 7.1). The main power on/off switch (circuit breaker) is located on the rear
panel and controls the AC power to the instrument.
The front panel contains a recessed toggle switch that distributes power within
the system console. This switch can be in the standby position (down) or on
position (up) and is equipped with a locking mechanism to prevent accidental
activation. The shaft of the switch must be pulled out to allow movement up or
down. The gas control valve is to the left of the toggle switch. For more details
on the gas control valve, see Gas Plumbing, page 33.
!
CAUTION
CAUTION! Placing the toggle switch in standby mode turns off the
ThermoCube cooling unit and camera power supply. All low voltage
equipment remains on.
31
7. Operating the IVIS® Spectrum
Status light
Bottom illumination
input optics
Gas valve
Standby toggle switch
Figure 7.1 IVIS Spectrum
Table 7.1 IVIS Spectrum status light modes
Status Light Is...
Startup Procedure
32
Indicates...
Blue
The system is in standby mode.
Blue and blinking
The CCD camera cooling system is disabled due to a power
interruption. Use the Living Image software to initialize the
system and enable the cooling system.
Red
The system is initializing or acquiring an image.
Green
The system is ready.
The same procedure is used to restart the system whether the shut down was
intentional or due to a power outage.
1. Confirm that the rear panel switch is off, the front panel toggle switch is in
the down position (in standby), and the power cord is plugged into the
console and the wall socket.
2. Turn on the computer and monitor, and start the Living Image® software.
3. Turn on the main power switch on the rear panel.
— The status light is blue.
4. Turn on the front panel toggle switch by pulling the toggle switch out and
up.
— The status light is blue and blinking.
IVIS® Spectrum Hardware Manual
5. In the Living Image software, click Initialize IVIS system in the IVIS
System Control panel (Figure 7.2).
— The status light is red.
6. Wait until the camera has cooled to operating temperature (requires about
ten minutes).
— The status light is green when the system is ready for operation.
You can monitor the system temperature in the Living Image software
(Figure 7.2).
The temperature square is red at startup and turns green
when the operating temperature is reached. Click the
temperature square to display the demand and measured
temperature of the CCD and stage.
Temperature box color indicates:
System not initialized.
System is initialized, but CCD temperature is out of
range.
System is initialized and CCD is at or within
acceptable range of the demand temperature (-90° C)
and locked. The system is ready for imaging.
Figure 7.2 IVIS System control panel in the Living Image software
The instrument is ready for operation when the camera temperature is locked at -90
C, as indicated by the green temperature square in the IVIS System Control panel.
For operating instructions, see the Living Image Software Manual.
7.2 Gas Plumbing
!
WARNING
WARNING! Use only isoflurane with the IVIS® Spectrum. DO NOT USE
FLAMMABLE ANESTHESIA GAS.
33
7. Operating the IVIS® Spectrum
!
CAUTION
CAUTION! Xenogen recommends using the XGI-8 Gas Anesthesia System
(Figure 7.3) when imaging small animals. This system supplies a controlled
amount of isoflurane to the imaging chamber and continuously reduces the
build-up of isoflurane in the chamber. If you want to use a gas other than the
recommended isoflurane/oxygen gas mixture or pure air, contact IVIS
Imaging Systems Technology Support.
Figure 7.3 XGI-8 Gas Anesthesia System
NOTICE
Be careful to use only tubing and other plumbing fixtures that do not fluoresce
or phosphoresce (glow) in the imaging chamber. Contact IVIS Imaging
Systems Technology Support for a list of acceptable materials.
The IVIS Spectrum is equipped with plumbing connections for supplying
isoflurane anesthetic gas to the imaging chamber and exhausting gases out. The
flow of gas into the imaging chamber is manually controlled by the gas valve
on the front panel of the console. The imaging chamber does not include a gas
scavenging system. This is provided by suitable auxiliary equipment such as the
XGI-8 Gas Anesthesia System.
Gas ports are located on the rear of the system console and are labeled GAS IN
and GAS OUT. GAS IN
means the direction of flow is into that port.
Similarly, the port labeled GAS OUT
means that flow can be exhausted out
of this port. The flow of gas from the rear panel GAS IN port is directed to the
main gas control valve on the front of the system and proceeds to a distribution
block located on the imaging platform where it exits through the port labeled
GAS.
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IVIS® Spectrum Hardware Manual
The mouse anesthesia manifold (Xenogen part no. 117706) with transparent
nose cones is provided with the IVIS® Spectrum. The manifold has capacity for
up to five mice and has a low profile (height) for operation with the system.
NOTICE
The IVIS Imaging System 100 Series and the XGI-8 Gas Anesthesia System
are equipped with a different five-station manifold (PN 118274) that is taller
than the low profile manifold supplied with the IVIS Spectrum. Only use the
low profile manifold with the IVIS Spectrum, which permits imaging the
smallest field of view at the highest platform height (FOV A).
The manifold port labeled GAS connects to the GAS port on the platform
distribution block. The anesthesia manifold also has a port labeled EXH that
should be connected to the EXH port on the platform distribution block. The
vacuum or exhaust circuit connects to the GAS OUT port on the rear of the
system console. No gases are exhausted from the imaging chamber unless gas
scavenging equipment such as the Xenogen XGI-8 Gas Anesthesia System is
used.
7.3 Door Operation
The door of the imaging chamber cannot be opened when the system status light
is red. The door is equipped with an electromagnetic lock to prevent accidental
opening during an imaging session.
!
WARNING
WARNING! Never try to defeat the door interlock or force the door open
during image acquisition when the status light is red. Such action could
expose the user to a severe pinch hazard from the rapidly moving imaging
platform.
7.4 Centering a Subject in the Field of View
!
WARNING
WARNING! The IVIS Spectrum is equipped with a Class II laser similar to the
type used for laser pointers (532nm, output < 1mW). Users are protected from
eye damage due to the eye’s physiological response to bright light by blinking.
A laser beam can be harmful to the eyes. ABSOLUTELY DO NOT look
directly into the laser beam when the laser is turned on. Be aware that laser
light can be easily reflected off of shiny surfaces and that this reflected light
can be dangerous. When the laser is on, remove all watches and jewelry
when placing hands in the imaging chamber.
1. In the Living Image software, choose the Enable alignment grid option in
the IVIS System Control panel (Figure 7.4).
35
7. Operating the IVIS® Spectrum
To display the alignment
grid, choose Alignment
Grid On.
Figure 7.4 IVIS System Control panel
2. Open the imaging chamber door to see the alignment grid projected by the
laser/galvanometer on the imaging platform (Figure 7.5).
— The field of view (FOV) setting defines the size of the squares in the
alignment grid.
3. To change the size of the squares in the alignment grid, select a different
FOV setting.
For example FOV settings, see Figure 6.4, page 24.
Figure 7.5 Laser alignment grid
NOTICE
The alignment laser turns off after 60 seconds. Repeat step 1 to turn on the
laser.
4. Using the alignment grid as a guide, place the specimen(s) at the locations
of interest, then proceed with image acquisition.
NOTICE
The Living Image® software controls the photographic illumination LED
intensity.
36
IVIS® Spectrum Hardware Manual
7.5 Fluorescent Imaging
The IVIS® Spectrum has both bioluminescent and fluorescent imaging
capabilities. To change from bioluminescent to fluorescent operation, select
fluorescence mode in the IVIS System Control panel and select an appropriate
excitation and emission filter (Figure 7.6).
Choose
luminescent or
fluorescent
imaging
Figure 7.6 IVIS System Control panel
A 150 Watt quartz tungsten halogen lamp with a dichroic reflector provides the
fluorescence excitation light. The Living Image® software controls the lamp
intensity level. The fluorescence light source is a module located behind the rear
panel and is not user serviceable.
The lamp output is delivered to the excitation filter wheel assembly located in
the rear compartment, where it is collimated and transmitted through a
fluorescence excitation filter. The 12 excitation filter wheel locations provide
10 fluorescence filters. Two filter slots contain a light block that is used during
bioluminescent imaging to prevent external light from entering the imaging
chamber. The 12-position excitation filter wheel is motorized and controlled
through the Living Image software.
Upon leaving the excitation filter, the light enters an optical switch that directs
the light to either the top of the imaging chamber or to the bottom of the stage.
When top illumination is selected, the light is equally split to four diffusers to
provide uniform stage illumination. When bottom or trans-illumination is
selected, the excitation light is directed to one of the 2 mm diameter holes in the
aperture plate located in the stage surface. This removable plate has a 15 x 23
array of 2mm diameter holes which provide discrete illumination locations that
are user selectable.
You can replace the array plate with a 3 mm thick glass plate or a well plate
holder to provide alternate illumination possibilities. A blank plate is also
provided for when the bottom illumination equipment is not in use. For more
details on how to use the top and bottom illumination systems, see the Living
Image Software User’s Manual.
Selecting an Emission
Filter
The IVIS Spectrum has a factory-installed filter set. The filters are mounted on
a rotating filter wheel and can be removed or replaced with filters with other
spectral characteristics. For more details, see Emission & Excitation Filter
Wheels, page 25. For information on how to replace a filter, see page 49. See
the Living Image® Software Manual for more details on how to select an
emission filter.
37
7. Operating the IVIS® Spectrum
Selecting an Excitation
Filter
In fluorescence mode, the excitation filter is automatically selected based on the
wavelength of the emission (imaging) filter selected from the IVIS System
Control panel. However, you can override the automatic selection in the IVIS
System Control panel.
The excitation filters are housed in a filter wheel module that is not accessible
to the user. Unlike the emission filters, the user cannot replace these filters.
Contact IVIS Imaging Systems Technology Support if an excitation filter must
be replaced.
7.6 Imaging Basics
Black Paper
Although the platform is black anodized, it is recommended that you image
items on a high quality black paper, especially biological specimens. Xenogen
Corporation has surveyed many types of paper and recommends Swarthmore,
Artagain, Black, part no. 445-109, size 8.5 inch x 11 inch. This paper prevents
illumination reflections and helps keep the stage clean.
Low Fluorescence Mat
When operating the system in the fluorescence mode, use the Low Fluorescence
Imaging Mat (Xenogen part no. 117839) to reduce background fluorescence.
The mat is made from a low reflectance textured plastic that is easily cleaned.
Glowing Materials
Always keep in mind that nearly EVERYTHING glows (that is, has the
potential to phosphoresce and contaminate the image). Most plastics, almost all
tape, plants, paint, rodent food (mostly plants), mouse urine, and animal
bedding have been found to glow.
Use caution when introducing materials into the IVIS® Spectrum. It is advisable
to prescreen all items by imaging them alone, before imaging them with
samples under study. Xenogen recommends using non-powdered gloves when
working with IVIS Spectrum equipment.
7.7 System Shut Down Procedure
Xenogen Corporation does not recommend power cycling the IVIS Spectrum
(turning the system components on and off) on a daily basis. Leaving the system
on keeps the camera cold and the electronics systems stable. The software is
preconfigured to run automatic background accumulation and self-diagnostics
overnight. The platform temperature and the computer monitor may be turned
off.
If it is necessary to shut down the system for any reason, such as long term
storage (more than 30 days), it is important to follow the procedure below.
1. Close the Living Image® software and save any information of interest at the
prompt.
2. Turn off the computer.
3. Turn off any gas to the imaging chamber and disconnect the gas supply if
necessary. If you are using the XGI-8 Gas Anesthesia system, follow the
shut down procedure in the XGI-8 Anesthesia System User Manual.
4. Turn off (standby) the toggle switch on the front of the IVIS Spectrum.
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IVIS® Spectrum Hardware Manual
5. Turn off the main circuit breaker switch located on the rear of the IVIS
Spectrum and disconnect the power cord from the wall socket.
6. Cover the gas inlet and outlet ports with masking tape to prevent entry of
dust.
7. Remove any loose objects from the imaging chamber and close the door.
If you plan to move the system, see important information on page 51.
If you have any problems during the shut down procedure, please contact IVIS
Imaging Systems Technology Support for assistance.
39
7. Operating the IVIS® Spectrum
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IVIS® Spectrum Hardware Manual
8 Troubleshooting
Measured Temperature Does Not Equal the Demand Temperature
Photographic Image Is Unacceptable . . . . . . . . . . . . .
Luminescent Image Is Unacceptable . . . . . . . . . . . . .
No Image Is Produced . . . . . . . . . . . . . . . . . . . . .
Hardware Problems . . . . . . . . . . . . . . . . . . . . . .
Accessing the Area Under the Imaging Platform . . . . . . .
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41
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42
43
44
44
46
8.1 Measured Temperature Does Not Equal the Demand Temperature
At start up, the Living Image® software programs the CCD camera to maintain
the CCD at -90° C. If the camera power supply and ThermoCube chiller unit
remain on, the system maintains this temperature regardless of whether the
Living Image software is open or the computer is turned on.
■
To check the temperature of the CCD, click the Temperature square (red or
green) in the Camera Control Panel of the Living Image software (Figure
8.1).
Click the temperature square to display
the demand and measured temperatures
of the camera and imaging chamber.
Figure 8.1 IVIS System control panel in the Living Image software
Problem
Possible Cause
Corrective Action
Measured temperature is
warmer than the demand
temperature.
A problem may exist with the
camera or the ThermoCube unit.
Contact IVIS Imaging Systems Technology
Support.
41
8. Troubleshooting
8.2 Photographic Image Is Unacceptable
Default camera controls are programmed during the initialization of the Living
Image® software. Changes to these settings can greatly affect the photographic
image. Check that the exposure time, f/stop and binning levels are set to the
default conditions.
If the f/stop is open too wide, the image may be saturated and less focused.
Binning to a higher value or increasing the exposure time can produce an image
that is too bright and lacks resolution. If you have questions about the
photographic settings, please contact IVIS Imaging Systems Technology
Support.
Also, refer to the Living Image Software Manual for further details on acquiring
images.
Problem
Possible Cause
Corrective Action
Image is streaked.
Subject moved during the
exposure.
Check to see if the subject may have moved. If the
subject is not on the sample stage, it is probably on the
floor of the imaging chamber. If the sample has moved,
locate and reanesthetize it. If gas anesthesia is being
used, confirm that the anesthesia is turned on and the
flow rate is appropriate.
Image is blurry.
Subject height is not correctly
entered in the Living Image
software.
If you are using the “Sample Height”setting to determine
the focus, confirm that the correct height of the sample
is entered. Alternatively, Auto-Focus may be used to
determine the sample height.
If you are using the “Manual”setting to determine the
focus, confirm that the focus has been manually
adjusted.
Incorrect f/stop setting.
The f/stop for photographs should be set to f/8. An f/stop
smaller than 8 reduces the depth of field in the
photograph.
An excessively moist
environment in the imaging
chamber can result in
condensation on the CCD
window (Figure 8.2).
Remove excess moisture in the imaging chamber and
allow it to thoroughly dry. Place a desiccant in the imaging
chamber to decrease the drying time.
Do not touch or let specimens touch the window at the
top of the imaging chamber. For more details on the care
of this window, see page page 48.
If the problem persists, contact IVIS Imaging Systems
Technology Support for assistance.
A white spot appears in the
center of the field of view.
Figure 8.2 Condensation example
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IVIS® Spectrum Hardware Manual
8.3 Luminescent Image Is Unacceptable
Binning, f/stop, and exposure time affect the appearance of a luminescent
image. Please refer to the Living Image® Software Manual for instructions on
setting binning, exposure time, and f/stop values.
In order to function properly and reduce camera noise, the CCD camera must
be cooled to the demand temperature before acquiring an image. If the camera
is not cooled to the demand temperature, imaging may result in false positive
signals.
Problem
Corrective Action
Light contaminationInternal
Check to see that there are no extraneous light sources in the imaging chamber. Many
substances phosphoresce when exposed to light. Be especially cautious of plastics and
substances that contain pigment. Be sure to pre-screen any substance or material before
performing actual experiments.
Light contaminationExternal
A 2” diameter high reflectance hemisphere can be special ordered to help check for light leaks
(XRH-1, Xenogen part number 118937, Figure 8.3).
To check for light leaks:
1. Place the high reflectance hemisphere in the imaging chamber on the stage using a subject
height of 3.5 cm at field of view D.
2. Take a luminescent image of the hemisphere using the luminescent settings: f/stop = 1,
Binning = Large (high sensitivity), and exposure time = 5 minutes.
If the hemisphere can be easily seen, there is a light leak. Contact IVIS Imaging Systems
Technology Support for assistance.
Camera noise
Verify that the camera is cooled to the demand temperature.
1. Check the measured temperature in the Camera Control panel to ensure that it is locked. If
the camera temperature is locked, the camera temperature box is green.
2. If the camera temperature box is red, click the red box to display the actual temperature. See
Measured temperature is warmer than the demand temperature., page 41.
Figure 8.3 High reflectance hemisphere
Wear powderless gloves when handling the hemisphere to prevent surface
contamination that may glow when using the IVIS® Spectrum.
43
8. Troubleshooting
8.4 No Image Is Produced
If no image is produced, there may be an error in the Living Image software, a
problem with the physical connections to the camera, or a hardware failure.
1. Close the Living Image® software and restart the computer.
2. Restart the Living Image software and try to acquire an image.
3. If after restarting the computer, you are still unable to produce an image,
contact IVIS Imaging Systems Technology Support for assistance.
8.5 Hardware Problems
Due to its integrated design, almost all of the components in the IVIS Spectrum
are not user-serviceable.
VOLTAGE
VOLTAGE! Do not remove any panel from the console, except for the filter
wheel access panel. Removing a panel could expose the user to hazardous
voltages.
If you suspect a problem with the instrument hardware, contact IVIS Imaging
Systems Technology Support.
Circuit Breaker (Power
Entry Module)
Under normal operation, the circuit breaker that is incorporated into the rear
panel power entry module (main power switch) should not trip. Frequent
tripping of this circuit breaker may indicate an underlying electrical problem. If
this occurs, unplug the system and contact IVIS Imaging Systems Technology
Support.
If the circuit breaker is occasionally tripped:
1. Confirm that the rear panel switch is off, the front panel toggle switch is
down (standby), and the power cord is plugged into the console and the wall
socket.
2. Turn on the computer and, if necessary, start the Living Image® software.
3. Turn on the rear panel main power switch.
— The status light is blue.
4. Turn on the front panel toggle switch by pulling the toggle out and up.
— The status light is blue and blinking.
5. Initialize the system.
— The status light is red and turns green when the system is initialized.
Wait until the camera has cooled to the demand temperature before you
begin an imaging session.
Computer Connections
Never disconnect or wiggle the rear panel computer connections when the
IVIS® Spectrum is powered on. If you suspect that any of the rear panel
computer connections are loose, follow the procedure below.
1. Close the Living Image software and shut down the computer.
2. Turn off the front panel toggle switch (standby).
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IVIS® Spectrum Hardware Manual
3. Turn off the rear panel circuit breaker switch.
Back panel
Front panel
Toggle switch
Figure 8.4 IVIS Spectrum, front and back panels
4. Check the connections between the computer and the rear panel of the IVIS
Spectrum. Tighten any loose connection.
5. Turn on the computer and start the Living Image software.
6. Turn on the rear panel main power switch.
— The status light is blue.
7. Turn on the front panel toggle switch by pulling the toggle out and up.
— The status light is blue and blinking.
8. Initialize the system.
— The status light is red and turns green when initialization is complete.
Wait until the camera has cooled to the demand temperature before you
begin an imaging session.
Laser/Galvanometer
The laser/galvanometer is not user-serviceable. If you notice any changes to the
alignment grid (for example, change in the brightness or position of the grid),
contact IVIS Imaging Systems Technology Support.
Lamp Replacement
The quartz halogen lamp that provides the fluorescent light source typically
operates for thousands of hours. This light source is housed in a compartment
that contains hazardous voltages and is not user accessible or serviceable.
VOLTAGE
VOLTAGE! DO NOT attempt to service the quartz halogen lamp.
If the lamp brightness is decreased or if lamp replacement is required, contact
IVIS Imaging Systems Technology Support.
45
8. Troubleshooting
8.6 Accessing the Area Under the Imaging Platform
During normal operation, the imaging platform always returns to the loading
position before the door can be opened. This helps ensure user safety and
convenience when loading subjects. Occasionally you may need access to the
area beneath the platform for cleaning or to retrieve a subject. Since the
platform moves rapidly and produces strong forces, the area beneath the
platform is not accessible unless the normal operation mode is overridden.
To access the space below the imaging platform:
1. Remove all items from the imaging stage that are taller than 2 cm and close
the door.
2. In the IVIS System Control panel, select Service from the FOV drop-down
menu.
— The platform moves to the service position and the door can be opened.
3. Place the stage lock down bar in position as shown in Figure 8.5.
Be careful not to disturb the platform heater cable or the gas tubing located
in this area.
To return the platform to the operating position:
1. Return the lock down safety bar to the stowed position under the front edge
of the imaging platform and close the door.
2. In the IVIS System Control panel, select Load from the FOV drop-down
menu.
— The imaging platform returns to the normal operating position.
The stage lock down bar is located at
the bottom front edge of the imaging
platform.
Pull the bar down from the stowed
position and place it as shown above.
Return the bar to its stow position
before returning the imaging platform to
the operating position.
Figure 8.5 Imaging platform and the stage lock down bar
If you need to access the space under the imaging platform, use the lock down bar as a safety precaution.
46
IVIS® Spectrum Hardware Manual
9 Care & Maintenance
Cleaning the IVIS Spectrum . . . . . . . . . . . . . . . . . . .
Cleaning the Lens Protection Window . . . . . . . . . . . . .
Changing the Emission Filters Used in Fluorescence Imaging
Moving the System . . . . . . . . . . . . . . . . . . . . . . . .
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47
48
49
51
9.1 Cleaning the IVIS Spectrum
If necessary, wipe the exterior surface of the IVIS Spectrum console with a soft
cloth. If the system requires more aggressive cleaning or sterilization, contact
IVIS Imaging Systems Technology Support.
!
DANGER
DANGER! DO NOT use sprays or fluids to clean the exterior or interior of the
module. Do not allow fluids of any kind to penetrate the electronics cabinet
under any circumstances. Sprays and liquids that come into contact with the
IVIS Spectrum may result in damage to the system or electrocution.
!
WARNING
WARNING! Do not use fluids or moistened towels to clean any part of the
system console where electrical or fiber optic cables make connections. Do
not use fluids of any kind near the filter wheel assembly. Turn off the electrical
power to the IVIS Spectrum by turning the front panel toggle switch to standby
and turning off the rear panel switch before engaging in cleaning operations
that use fluids. The imaging chamber power switch is located on the rear of
the system console.
Approved Cleaning
Solutions
The compounds shown in Table 9.1 do not damage the internal finish of the
IVIS® Spectrum imaging chamber and are suitable as cleaners, if required. Do
not use any solution not included in this list. In particular, avoid strong bases,
bleach, or acids that may potentially damage the unit and compromise its
operation.
!
IMPORTANT
ALERT! Do not spray cleaning solutions in the imaging chamber.
47
9. Care & Maintenance
Table 9.1 Acceptable cleaning solutions for the IVIS® Spectrum imaging chamber
Cleaning Solution
Cidexplus®
Manufacturer
Solution (3.4% glutaraldehyde) Johnson & Johnson Medical
70% methyl alcohol/30% deionized
water solution
70% ethyl alcohol/30% deionized water
solution
Sporicidin® Sterilizing Solution (1.56%
phenol)
Sporicidin International
Clidox-s® Disinfectant
Pharmacal Research Laboratories, Inc.
NOTICE
Xenogen makes no claims as to the sterility of the IVIS imaging chamber after
using the solutions in Table 9.1. Please refer to the manufacturer’s literature
for information as to the applicability of the compound for the organism of
interest. Consider dedicating an imaging system for immunodeficient animals.
This will reduce the risk of cross contamination.
Cleaning the Imaging
Platform
It is recommended that you use a lint-free wipe, such as Scott Pure® wipe or a
Kaydry EX-L® wipe to minimize the presence of particulate matter in the
imaging chamber.
After saturating a lint-free wipe, clean the internal surfaces using a gentle
circular motion. Do not pour or spray the solution directly onto internal
surfaces. Rinse surfaces using a wipe saturated with sterile deionized water. Do
not allow puddles of water to remain on the surfaces.
Avoid cleaning the ceiling of the image chamber except when necessary. When
cleaning the chamber ceiling, use extreme care to avoid damaging the lens
protection window and the light diffusers in this area.
To avoid any phosphorescence from the cleaner, be sure that the surfaces are
dry before using the imaging chamber.
9.2 Cleaning the Lens Protection Window
Contact IVIS Imaging Systems Technology Support for information about
cleaning or sterilizing any of the optical components or replacing the optical
filters. A clear window at the top of the imaging chamber protects the lenses and
other optical components from contamination. Do not touch or allow subjects
to touch this window, otherwise image quality may be impaired. Further, the
glass window could be cracked or broken if struck by a hard object. The lens
protection window should be cleaned periodically.
To clean the surface of the lens protection window:
1. Blow off any accumulated dust using a Dust-Off® compressed gas duster.
2. Dampen a lint-free cloth with isopropyl alcohol and gently swab the
window surface to remove any smudges or streaks.
3. Repeat step 2 using a clean area of the cloth or a new cloth until the window
is clean.
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IVIS® Spectrum Hardware Manual
4. Take an image to confirm that the image quality is restored.
9.3 Changing the Emission Filters Used in Fluorescence Imaging
The following steps explain how to change one or more of the optional emission
filters used for fluorescence imaging in the IVIS® Spectrum.
Before you begin, gather the following tools:
• 5/32″ hex key
• #2 Phillips head screw driver
• One pair of lint free, powderless gloves
1. In the Living Image® software, save any important data, then exit the
program.
2. Turn the front panel switch to off (standby) and turn off the main power at
the rear of the system console.
3. Using the 5/32″ hex key, remove the eight screws that hold the filter access
panel in place on the upper right side of the console.
— The panel is held in place by four pins until it is pulled forward. be
careful not to damage the light-sealing gasket when removing the panel.
NOTICE
The panel has a machined recess on the inner left-hand side (towards the
front of the console). Noting this orientation is helpful when you reinstall the
panel.
4. Notice that the 1/2″ shaft on the right side of the filter wheel compartment
has a carriage clamp lock with a handle. Turn the handle counter-clockwise
to loosen the clamp so that it can slide freely over the shaft.
NOTICE
Starting with step 5, wear lint-free, powderless gloves to prevent smudging
the filters.
5. Grip the filter wheel by the metal rim and pull the filter wheel carriage
forward until it stops. Be careful not to touch the glass filters or to dislodge
the two drive belts.
Since the carriage slides out about eight inches, only two or three of the
filters will be visible. The filter wheel holds the filters numbered 1-11.
6. To remove a filter from the filter wheel:
a. Slowly rotate the filter wheel until the filter of interest is exposed.
b. Use a #2 Phillips head screw driver to loosen the flat head screw ONE
TURN.
The screw has a captured O-ring that retains the glass emission filter.
c. Use two gloved fingers to remove the filter. Alternatively, gently push
the filter up with a gloved finger.
49
9. Care & Maintenance
7. To clean a filter, use a Dust-Off® compressed gas duster to apply an air
stream at an angle to the filter surface, otherwise dust particles could be
driven into the filter coating.
NOTICE
Do not blow air onto a filter in the filter wheel as dust could be spread to other
optical components.
8. To install a filter, insert the filter into the filter wheel recess and tighten the
O-ring screw until it bottoms out (about one turn).
NOTICE
Make sure that the filter retaining screw and O-ring are fully seated to prevent
interference or rubbing against components above them. The top of the filter
metal ring should be nearly flush with the wheel. The filter encapsulating metal
ring has one side that is thinner than the other. The thin part of the ring should
face downward in the wheel recess so that the arrow that is printed on the side
of the filter points up.
9. Push the filter wheel carriage back into the console by placing one finger on
the rim of the upper filter wheel.
10. While you hold the carriage in the closed position, turn the carriage-locking
clamp in the clock-wise direction. Be careful not to dislodge any drive belts
off of their pulleys when locking the clamp.
11. Replace the access panel by positioning it onto the four pins. The inner
machined out recess should be on your left side.
12. Loosely install the eight screws, then lightly tighten the four central screws
and in a crisscross pattern. Lightly tighten the four outer screws in a
crisscross pattern.
NOTICE
Do not over tighten the screws. The panel is designed to seal out light without
large clamping force.
13. Restart the system following the procedure on page 31.
NOTICE
If you change a filter or a filter position, you must also change the
corresponding filter label in the Living Image® software. For more details, see
the Living Image Software Manual.
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IVIS® Spectrum Hardware Manual
9.4 Moving the System
Removing the Dome
It may be necessary to remove the plastic camera dome when you move the
IVIS® Spectrum though a doorway or other low overhang.
■
Moving the System
To remove the dome, wrap a flat blade screw driver with a thin cloth or
paper towel and use the screw driver to gently pry the dome off at the rear
of the console.
If the console is only repositioned a short distance within the range of the power
cord and cables, the power need not be turned off. However, be careful not to
accidentally turn off the power when gripping the system at the right rear.
When possible, two people should move the IVIS® Spectrum. The console is tall
and it is difficult to see potential obstacles when pushing the console from
behind. Make sure that the path is clear of items that could jolt the casters and
cause the console to tip. Move the console slowly so that you are always in
control of its motion.
To move the system:
1. Put the front panel toggle switch in the standby position.
2. Turn off the main power switch on the rear panel and disconnect the power
cord from the wall socket and the console.
3. Remove all cables from the rear panel of the console.
!
CAUTION
CAUTION! Any cables that dangle from the console could cause the system
to topple if caught by a caster.
4. Unlock the four casters.
5. Grip the console at a convenient location, but do not use the door handle as
a grip. Be careful when gripping the right rear of the console so that you do
not damage the connectors or fan grills.
6. After the system is positioned at the new location, lock the casters.
7. Reconnect the cables and power cord.
8. If necessary, reinstall the dome.
9. To restart the system:
a. Confirm that the rear panel switch is off, the front panel toggle switch is
down (standby), and all cables are reconnected.
b. Confirm that the power cord is plugged into the console and the wall
socket.
c. Turn on the computer and start the Living Image® software.
d. Turn on the rear panel main power switch.
— The status light is blue.
e. Turn on the front panel toggle switch by pulling the toggle out and up.
— The status light is blue and blinking.
f. Initialize the system.
51
9. Care & Maintenance
— The status light is red.
The status light turns green when the system is initialized. Wait until the
camera has cooled to the demand temperature before you begin an
imaging session (about ten minutes).
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IVIS® Spectrum Hardware Manual
10 Servicing & Maintaining the ThermoCube Chiller Unit
Required Tools . . . . . . . . . . . . . . . . . .
Turning Off Electric Power to the Main Console
Removing the Console Rear Panel . . . . . . .
Sliding the Tray Out . . . . . . . . . . . . . . .
Filling the ThermoCube Unit . . . . . . . . . . .
Reassembling the System . . . . . . . . . . . .
Restarting the IVIS Spectrum . . . . . . . . . .
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Over time, the coolant level in the ThermoCube thermoelectric chiller unit may
diminish and require replenishing. It is recommended that you check the coolant
level in the ThermoCube unit every six months. This chapter explains how to
add coolant to the Thermocube unit.
10.1 Required Tools
The following tools and items are required, but not provided.
• Allen Wrenches (hex head wrench): 3/32" and ¼" measured across the
flats (AF).
• Measuring cup with a spout.
• Spectral TE Camera Cooling Mixture (Xenogen part no. 119219, includes
a 3/32" and ¼" allen wrench) or a solution of 25% pure ethanol, 75%
distilled water.
• Paper towel or cloth for clean up.
10.2 Turning Off Electric Power to the Main Console
VOLTAGE
VOLTAGE! Do not remove any panels from the console until you turn off the
IVIS Imaging System disconnect the power cord. Otherwise, electric shock
could result.
1. Pull the front toggle switch out and down to the standby position.
2. Turn off the main power switch on the rear panel and disconnect the console
power cord from the wall socket.
3. Move any cables away from the lowest rear panel of the console so that the
panel can be removed (Figure 10.1). (No other cables need to be removed.)
53
10. Servicing & Maintaining the ThermoCube Chiller Unit
Figure 10.1 Lower rear panel
Note that the power cord has been removed from the console.
10.3 Removing the Console Rear Panel
1. Use a 3/32" hex head wrench to remove the screws that secure the bottom
rear (fan) panel (Figure 10.2).
2. Carefully remove the panel and lean it against the side of the console.
!
CAUTION
CAUTION! Be careful not to pull too hard on the rear fan panel, otherwise its plug
may become disconnected. If this happens, contact IVIS Imaging Systems
Technology Support).
.
ThermoCube unit
Figure 10.2 Removing the lower rear panel using a hex head wrench (left) and ThermoCube unit exposed (right)
54
IVIS® Spectrum Hardware Manual
10.4 Sliding the Tray Out
The ThermoCube unit sits on a tray that must be pulled out to access the unit.
1. Use the ¼" hex head (Allen) wrench to loosen and remove the bolt that
secures the tray under the ThermoCube unit to the console (Figure 10.3).
Figure 10.3 Loosen the tray locking bolt
2. Move any cables or tubes out of the way so that the tray can be pulled out
freely.
3. Pull out the tray with the ThermoCube unit (Figure 10.4).
NOTICE
The ThermoCube unit has its own power switch located at the rear of the unit.
The power switch is not visible until the unit is pulled out. Make sure that the
power switch is not accidentally turned off when you slide the tray out or back
into the console.
4. Remove the fill cap on the ThermoCube chiller and check coolant level. If
the coolant level is half way up the neck of the tank, no additional
coolant is required.
Figure 10.4 Removing the fill cap
55
10. Servicing & Maintaining the ThermoCube Chiller Unit
10.5 Filling the ThermoCube Unit
1. If additional coolant is required, wrap a piece of cloth or a paper towel
around the neck of the tank to absorb accidental spills (Figure 10.5).
2. Using a spouted measuring cup, add small quantities of the 25% ethanol,
75% distilled water solution to the ThermoCube tank until the coolant level
is half way up the neck of the tank. Use care not to spill the liquid and wipe
any liquid from the top or walls of the chiller.
!
CAUTION
CAUTION! DO NOT OVERFILL the ThermoCube unit. Over filling the tank
can cause fluid to run down the chiller walls and contact the power plug.
Figure 10.5 Adding coolant to the ThermoCube unit
NOTICE
If the Xenogen Spectral TE Camera Cooling Mixture (Xenogen part no.
119219) is not available, a suitable substitute can be made from ordinary 80
proof Vodka and distilled water. To make 750 ml of the disinfecting coolant,
mix 470 ml of 80 proof alcohol (40% ethanol) and 280 ml distilled water.
!
WARNING
WARNING! If you prepare your own ethanol solution, adhere to the 25% (by
volume) pure ethanol concentration. A higher alcohol concentration may be
flammable and a lower concentration may not suppress organic growth in the
cooling system or camera.
56
IVIS® Spectrum Hardware Manual
10.6 Reassembling the System
1. Replace the cap on the ThermoCube unit. Make sure that the cap is fully
tightened to prevent rapid coolant evaporation.
2. Slide the tray back into the console while you hold the cables and hoses out
of the way to prevent snagging.
3. Replace the tray bolt using the ¼" hex head (Allen) wrench (Figure 10.3).
4. Confirm that the ThermoCube power switch is on and that no tools or other
hardware remain inside the IVIS.
5. Replace the lower rear panel using a 3/32" hex head wrench to tighten the
screws (Figure 10.2).
10.7 Restarting the IVIS Spectrum
1. Confirm that the IVIS system rear panel power switch is off and that the
front panel toggle switch is in the down (standby) position.
2. Reconnect the power cord first to the console and then to the wall socket.
3. Confirm that all other cables are properly connected.
4. Turn on the computer.
5. Turn on the rear panel power switch.
— The front status light is blue.
6. Turn on the front panel toggle switch by pulling out and up.
— The status light is blue and blinking.
7. Start the Living Image software and initialize the system.
— The status light is red and turns green when the system is initialized.
Wait until the camera has cooled to the demand temperature before you
begin an imaging session (about 10 minutes).
57
10. Servicing & Maintaining the ThermoCube Chiller Unit
[This page intentionally blank.]
58
IVIS® Imaging System 200 Series Hardware Manual
Appendix A Spare Parts List
Description
Xenogen Part Number
Mouse Inhalation Nose Cone
117150
Low Profile, 5 Station Manifold
117706
XRH-1 High-Reflectance Hemisphere with container
118937
Black Paper, Swarthmore, Artagain. 9x12”, 24 sheet pad
117837
Low Fluorescence Imaging Mat, 11.6” square
117839
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A. Spare Parts List
[This page intentionally blank.]
60
IVIS® Spectrum Hardware Manual
Index
A
accessory cable 27
back connector 28
stage connector 28
anesthesia delivery system 34
anesthesia manifold 35
array plate 37
B
back connector 28
base enclosure 26
black paper 38, 59
burn hazard 11
C
camera noise 43
caution definition 5
CCD camera 22
specifications 17
centering a subject 35–36
changing emission filters 49–50
chemical & biological safety 14
chiller unit 22
circuit breaker 44
cleaning
lens protection window 48–49
system 9
cleaning solutions 47, 48
computer 27
connections 44
crash avoidance system 26
D
definitions
caution 5
voltage 5
warning 5
dome 51
door operation 35
E
electrical power requirements 17
electrical safety 11
emission filter
selecting 37
emission filter wheel 25
emission filters
changing 49–50
environmental considerations 7
heat 7
location of system 7
space and ventilation 8
water and moisture 8
environmental requirements 18
excitation filter
selecting 38
excitation filter wheel 25
eye safety 11
F
field of view
centering a subject 35–36
filter wheel 25
filter wheel access panel 14
fluorescence imaging
changing emission filters 49–50
fluorescence imaging components specifications 18
fluorescent imaging 37
emission filter 37
excitation filter 38
G
gas in/out 34
gas plumbing 33
glowing materials 38
H
heat safety 7
high reflectance hemisphere 29, 43, 59
I
image
blurry 42
streaked 42
imaging chamber 23
door 35
61
Index
gas plumbing 33
imaging platform
accessing space below 46
lock down bar 46
warming system 25
imaging system
proper location 7
information on manual instructions 6
instrument labels
caution 6
pinch hazard 6
read manual 6
isoflurane gas anesthesia 33
L
lamp 45
laser caution label 6
laser safety 7
laser/galvanometer 26, 45
lenses 24
light contamination 43
lightning 8
limited warranty 15–16
location for imaging system 7
location safety 7
low fluorescence imaging mat 59
low fluorescence mat 38
low profile manifold 59
luminescent image unacceptable 43
M
mat, low fluorescence 38
measured unequal to demand temperature 41
mechanical safety 12
moving the system 9, 51–52
N
no image produced 44
nose cone 59
O
optical components
lenses 24
optical specifications 18
other equipment, chemicals, or supplies 9
62
P
patents 16
photographic image unacceptable 42
pinch hazard label 6
power cord 8
power line surges 8
power outages 9
power overloading 9
power sources 8
Q
quartz halogen lamp 45
R
read manual label 6
restarting the system 31–33
S
safety
burn hazard 11
chemical & biological 14
electrical 11
eye 11
laser 7
mechanical 12
water and moisture 8
servicing system 10
servicing the ThermoCube unit 53–57
shut down procedure 38–39
space and ventilation safety 8
spare parts 59
black paper 59
low fluorescence imaging mat 59
low profile manifold 59
nose cone 59
XRH-2 high reflectance hemisphere 59
specifications
CCD camera 17
electrical power requirements 17
environmental requirements 18
fluorescence components 18
optics 18
weights and dimensions 19
stage connector 28
starting the system 31–33
status lights 31–32
IVIS® Spectrum Hardware Manual
system
cleaning 9
moving 9, 51–52
servicing 10
shut down 38–39
T
temperature
measured unequal to demand 41
ThermoCube
servicing 53–57
ThermoCube chiller unit 22
trademarks 16
transillumination 37
troubleshooting
camera noise 43
light contamination 43
measured temperature does not equal demand
temperature 41
no image produced 44
streaked image 42
unacceptable luminescent image 43
unacceptable photographic image 42
V
voltage definition 5
W
warming system 25
warning definition 5
water and moisture safety 8
weights and dimensions 19
X
XGI-8 Gas Anesthesia System 34
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Index
64