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Prep-ahead Item Description Use Prep-ahead Yes or No If prep-ahead is used, the AutoSampler will begin the sampling process after the prep-ahead timer has expired. This allows you to sample for extended lengths of time, while the previous sample chromatogram is running, without adversely affecting the throughput of your system. The prep-ahead timer starts after the Auto Sampler has finished all injection actions for the previous injection. Prep-ahead Delay 0 to 999.99 minutes Adjust this time so that sample adsorption is complete just as the GC becomes Ready following the previous sample run. Sampling Item Description Sample Depth (%) 0 to 100% Sample depth determines whether the sample is adsorbed from the liquid sample or from the headspace above the sample. For liquid sampling, carefully adjusting the sample depth so that the fiber holder does not contact the sample liquid prevents sample carryover. Agitation Strokes 0 to 500000 If Headspace sampling is used, no agitation is required. Enter a value of 0. For liquid sampling, agitation is generally desirable to speed adsorption of the sample. Assume approximately 100 agitation strokes per minute of adsorption required. 1,000 agitation strokes is typical. Adsorb Time 0 to 999.99 minutes The adsorb time is executed for each Agitation Stroke. For Headspace sampling or liquid sampling without agitation, enter the total desired adsorption time, for example 10.0 minutes, divided by the number of agitation strokes, typically 1. For liquid sampling with agitation, enter a value of 0, which will allow the maximum agitation rate. 450-GC Sample Delivery 259