Download Cyto Pulse Sciences, Inc.
Transcript
PA-4000 User Manual Ch5: rev.1-1/05 5.3.3 Reviewing the Last Protocol Log and Log History The Last Protocol Log is now ready for viewing and saving. It provides a record of the protocol that was last delivered to the test sample. A description of Last Protocol Log for this basic example is given in Figure 5-3. A representation of the actual pulse-train is shown below in Figure 5-4. The parameters for every pulse delivered are recorded. Note that the SetV and MonV parameters are referring to the voltage of the HVPS and reservoir capacitor, not the pulse amplitude. Each time the protocol is run, the Last Protocol Log is refreshed, and the previous results are added to the Log History. The Log History is the record of the entire electroporation Protocol Information: Filename (if applicable) Date: MM-DD-YY-DoW Time: 24-Hour Clock System Mode Protocol File: BASICTEST.PRO 12-17-2004-6 18:51:35 Mode: Electroporation Delivered Pulse Parameters subdivided by Group number where: GRP = Pulse Group # NUM = Pulse # in Group WIDTH = Pulse Duration INTVL = Time between pulses SetV = Set HVPS Voltage MonV = Measured HVPS Voltage >GRP >1 >1 NUM 1 2 WIDTH 0.020 0.020 INTVL 0.20 0.20 SetV 500 500 MonV 500 500 >GRP >2 >2 >2 NUM 1 2 3 WIDTH 0.100 0.100 0.100 INTVL 0.30 0.30 0.30 SetV 200 200 200 MonV 205 205 205 Load Estimator Result >Estimated load > 19 ohms Calculated Conductance >Estimated conductance < 0.053 siemens System Message >Normal Completion $ Figure 5-3: The Last Protocol Log for the Basic Test Run session that has occurred since the software was started. It can be viewed by clicking the Show Log History button. The Log History may then be saved as a .txt file by clicking File>Log Save As or the Notebook button. The Log may be printed to file or by a printer by selecting the Printer button. Figure 5-4: The Pulse-Train Delivered by the Basic Protocol Cyto Pulse Sciences, Inc. P. O. Box 609, Columbia, MD 21045, Voice: 410-787-1890 5-9