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PALM® MicroLaser Systems
Protocols
Specimen isolation and collection................................
8
Laser cutting (microdissection) of the samples ...........................
8
• Tricks for improvement of the morphology sight of sections
o Ethanol
o Glycerol in PBS
Laser Pressure Catapulting of the samples .................................
9
Looking into the cap to see the catapulted samples ....................
9
Getting the collected cells from the cap into the tip of the tube.....
9
Downstream Applications ............................................ 10
DNA ..........................................................................................
10
Preparation of DNA from catapulted samples..............................
10
RNA ..........................................................................................
11
Some special tips for working with RNA .....................................
11
Preparation of frozen sections ..................................................
11
Preparation of RNA from catapulted samples ..............................
12
o
Proteinase K digestion
Chromosome preparation on request........................................
14
Isolation of living cells on request............................................
14
Protocols_EN_0403.doc
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