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CHAPTER 1 WE L C O M E MiraiBio MasterPlexTM QT 2.0 Welcome to the MiraiBio MasterPlexTM QT User Manual. MasterPlex QT software analyzes results files (*.csv) from the LuminexTM 100 system. 1.1 About This Manual This manual explains how to use the MasterPlex QT 2.0 software to: • import results files (*.csv) from the Luminex 100 system • designate standard, unknown, control, and background wells • generate standard curves • compute analyte concentrations • generate data charts and reports What’s New in MasterPlex QT 2.0 MasterPlex QT 2.0 offers new features, including the ability to: • apply a standard from one plate (source plate) to compute the concentrations in another plate (destination plate) • view a residual plot for standard curves, specify outliers in a standard curve, or apply weighting to a standard curve • use unknown samples that are diluted prior to the assay and analysis • create a virtual plate (a software simulation of a plate) that contains data from more than one actual plate (.csv or .mlx) so that results across multiple plates can be compared and analyzed • generate a Dose-Reponse curve and determine the logEC50 value Conventions Used in This Manual This manual describes the steps required to perform the various tasks associated with the MasterPlex QT software. The manual uses a step format to explain the various tasks associated with MasterPlex QT. The symbol may follow a step instruction. It indicates the software response to the action performed by the user. MasterPlex QT www.miraibio.com 1.1