Download Mandatory qualifiers

5' sequence matches:
From 43 to 61 with 0 mismatches
3' sequence matches:
From 183 to 199 with 0 mismatches
Sequences output to file:
from 62 to 182
tcgagagccgtattgcgatatagcgcacatgcgttggacacagatgagca
cacagtgacatgagagacacagatatagagacagatagacgatagacaga
cagcatatatagacagatagc
sequence trimmed from 5' end:
TCTAGAACCGGTGACGTCTCCCATGGTGAAGCTTGGATCCACGATATCCT
GCAGGAATTCC
Sequence: pTYB1.seq
Vector: pTYB1
5' sequence matches:
From 40 to 58 with 0 mismatches
3' sequence matches:
From 180 to 196 with 0 mismatches
Sequences output to file:
from 59 to 179
tcgagagccgtattgcgatatagcgcacatgcgttggacacagatgagca
cacagtgacatgagagacacagatatagagacagatagacgatagacaga
cagcatatatagacagatagc
sequence trimmed from 5' end:
CTTTAAGAAGGAGATATACATATGGCTAGCTCGCGAGTCGACGGCGGCCG
CGAATTCC
sequence trimmed from 3' end:
TCGAGGGCTCTTCCTGCTTTGCCAAGGGTACCAATGTTTTAATGGCGGAT
Sequence: pTYB1.seq
Vector: pBS_KS+
No match
Sequence: pTYB1.seq
Vector: pLITMUS
No match
% more pbluescript.fasta
>pBlueScript_from_84_to_204 KS+
tcgagagccgtattgcgatatagcgcacatgcgttggacacagatgagcacacagtgaca
tgagagacacagatatagagacagatagacgatagacagacagcatatatagacagatag
c
>litmus.seq_from_62_to_182
tcgagagccgtattgcgatatagcgcacatgcgttggacacagatgagcacacagtgaca
tgagagacacagatatagagacagatagacgatagacagacagcatatatagacagatag
c
>pTYB1.seq_from_59_to_179
tcgagagccgtattgcgatatagcgcacatgcgttggacacagatgagcacacagtgaca
tgagagacacagatatagagacagatagacgatagacagacagcatatatagacagatag
c
2. Running vectorstrip allowing maximum 30 percent mismatch, then asking only for best hits:
% vectorstrip litmus.seq
Strips out DNA between a pair of vector sequences