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[ Team LiB ] distmat distmat calculates the evolutionary distances between every pair of sequences in a multiple alignment. The sequences must be aligned before running this program. Here is a sample session with distmat: % distmat pax.align Creates a distance matrix from multiple alignments Multiple substitution correction methods for proteins 0 : Uncorrected 1 : Jukes-Cantor 2 : Kimura Protein Method to use [0]: 2 Output file [outfile.distmat]: Mandatory qualifiers (bold if not always prompted): [-msf] (seqset) File containing a sequence alignment. -nucmethod (menu) Multiple substitution correction methods for nucleotides. -protmethod (menu) Multiple substitution correction methods for proteins. [-outf] (outfile) A name for the distance matrix. Optional qualifiers (bold if not always prompted): -ambiguous (boolean) Use the ambiguous codes in the calculation of the Jukes-Cantor method, or if the sequences are proteins. -gapweight (float) Weight gaps in the uncorrected (nucleotide) and Jukes-Cantor distance methods. -position (integer) Choose base positions to analyse in each codon e.g., 123 (all bases), 12 (the first two bases), 1, 2, or 3 individual bases. -calculatea (boolean)