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Download MIT1000 User`s Manual - Micro Imaging Technology, Inc.
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Step 5: Prepare the Micro-Centrifuge Tube Suspension Items Needed Inoculating loop containing organism cells Micro-centrifuge tube from Step 3 Procedure While holding the inoculating loop that contains the organism cells: 1. Hold the water-filled micro-centrifuge tube in one hand. 2. Open the tube and tilt it no more than 45º making sure not to spill the contents. 3. Insert the inoculating loop into the water in the microcentrifuge tube and dislodge the sample into the water by agitating the loop in the water as shown in Figure 24 – Dislodge the Organism Cells into the Water in the MicroFigure 24. centrifuge Tube 4. Withdraw the loop and immediately close the tube. 5. Properly dispose of the contaminated loop in a Biohazard Bag. Step 6: Vortex the Sample Items Needed Micro-centrifuge tube with the organism cells in dilution from step 5. Vortex mixer Procedure 1. Vortex the micro-centrifuge tube for at least 20 seconds, Figure 25. 2. Proceed directly to Step 7. Note: To ensure optimum performance, do not delay proceeding to Step 7. Figure 25 – Vortex the Microcentrifuge Tube MIT 1000 System User Guide V3.5
