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Voyager User’s Guide 12. If the compound peak is the only peak within the retention time window, click Update or double-click on the peak to open the Update dialog. If the information in the box is not correct, enter the correct data and click OK. OR If the compound peak is not within the retention time window or if there are other peaks in the retention time window, drag the center, blue, dashed line to the desired peak and the retention time will be updated automatically. 13. Repeat this procedure for each library compound. 14. Select Re-integrate from the Analyzer menu. Each compound should be correctly identified. 15. If you are using another column for your application, repeat this procedure for the next column. 16. Save the assay and then send it to Voyager. 17. Using SiteChart, switch to the Instrument tab and run an analysis on each column. 18. Ensure all compounds are correctly identified and then use these analyses to calibrate Voyager. Updating the Calibration Curve After you have updated the compound retention times you can also update the calibration curve for each library compound. It is not necessary to update the calibration curves, but multiple point calibration will provide the most accurate response. 1. In the SiteChart software, select the Assay tab. 2. Select the column/detector combination required for your compound. Ensure your compound is already listed in the library. 278
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