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CHAPTER 16. PROTEIN ANALYSES
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Figure 16.25: Setting parameters for proteolytic cleavage detection.
• Min. and max. fragment length Likewise, it is possible to limit the output to only display
sequence fragments between a chosen length. Both a lower and upper limit can be chosen.
• Min. and max. fragment mass The molecular weight is not necessarily directly correlated
to the fragment length as amino acids have different molecular masses. For that reason it
is also possible to limit the search for proteolytic cleavage sites to mass-range.
Example!: If you have one protein sequence but you only want to show which enzymes cut
between two and four times. Then you should select "The enzymes has more cleavage sites than
2" and select "The enzyme has less cleavage sites than 4". In the next step you should simply
select all enzymes. This will result in a view where only enzymes which cut 2,3 or 4 times are
presented.
Click Next if you wish to adjust how to handle the results (see section 9.1). If not, click Finish.
The result of the detection is displayed in figure 16.26.
Figure 16.26: The result of the proteolytic cleavage detection.