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This requires that all observations have been made at the same equidistant intervals all over the lake. If relatively more observations have been made in dense near-shore macrophytes, the observations should be weighted before RPA is calculated. Laboratory work Before filtering for Chl a, absolute conductivity, temperature (°C) and pH is measured (accuracy 1 µS and 0.1 pH unit, respectively). This is done in the field. For chlorophyll measurements 2 litre in each lake is filtered (exact volume in ml is recorded) through a 47 mm GF/C filter. The filter is folded, placed in a test tube, wrapped in alufoil, put in a zip plastic bag marked with lake name, date and filtered volume (ml) and frozen (-20 °C) until analyses. DOC. A 100 ml subsample of the filtered water is kept dark and cold (<5 °C) in an acid washed and well rinsed bottle. Use gloves to avoid contamination, when handling the water. DOC is analysed at the University of Copenhagen. Water samples for water chemistry are frozen until analyses. Zooplankton and phytoplankton samples are stored in darkness – not frozen. These samples are send to NERI, Silkeborg (Aarhus Universitet, Danmarks Miljøundersøgelser, Sø-sektionen, Vejlsøvej 25, 8600 Silkeborg) following the last sampling. Equipment for monthly field work • A rubber dinghy with oars, outboard and anchor • Pump for the dinghy • Plastic bottles for sediment (two per lake, only during the first and last visit) • A water sampler • 30 litre tub • Zooplankton filter • Plastic funnel for 50 ml zooplankton bottles • Squeeze bottle • 2 x 50 ml glass bottles including 2% lugols solution for zooplankton and phytoplankton samples • Plastic tubs for water chemistry (200 ml) and chlorophyll a (2 litre) • Secchi disc • Optic shuttle and Tidbit Coupler for the StowAway Tidbit Temp Logger • Depth sounder with thermometer • Life jacket • Survival suit • Data form Appendix 6A. Equipment for annual vegetation sampling • • • • 50 Water glass Plant rake with robe Data form Appendix 6B. GPS navigator.