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4 Searching Databases Performing Searches • Single-protein search: See Chapter 6, “Using the Sequence Gazer to Search for Single Proteins.” • Gene-restricted absolute mass search: See “Searching for Gene-Restricted Absolute Masses” on page 146. • Gene-restricted biomarker mass search: See “Searching for Gene-Restricted Biomarkers” on page 152. 3. Click Save. Note Altering search parameters has no effect on searches already added to MS/MS experiments. Edit Predefined Search Dialog Box Parameters The parameters in the Edit Predefined Search dialog box depend on the type of search that you select in the Search Type list: • Absolute Mass: See “New Search in Experiment X Dialog Box Parameters for Absolute Mass” on page 123. • BioMarker: See “New Search in Experiment X Dialog Box Parameters for Biomarkers” on page 134. • Sequence Tag: See “New Search in Experiment X Dialog Box Parameters for Sequence Tags” on page 142. • Single Protein: See Chapter 6, “Using the Sequence Gazer to Search for Single Proteins.” • Gene-Restricted Absolute Mass : See “Searching for Gene-Restricted Absolute Masses” on page 146. • Gene-Restricted BioMarker: See “Searching for Gene-Restricted Biomarkers” on page 152. Running a Predefined Search You can run a predefined search by using either of the following procedures. To run a predefined search with the Run Search command 1. Select all the desired experiments in the data grid. • To help you sort entries in the data grid, you can click the title row of the column to sort entries from lowest to highest value or highest to lowest. • To select contiguous experiment names, click the name of the first experiment, hold down the SHIFT key, and click the last experiment name that you want to select. • To select noncontiguous experiment names, click the name of the first experiment, hold down the CTRL key, and click each separate experiment name. 108 ProSightPC User Guide Thermo Scientific