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Normalization Relative to Positive Controls Across Experiments This is done by dividing the values in the dataset by the median value of the positive controls across all samples. For example: Gene i sample j /median (all positive control genes across all samples) Gene i sample k / median (all positive control genes across all samples) Refer to the above image. Actions 1. Click a complete dataset in the Experiments navigator. The item is highlighted. 2. Click the Normalize toolbar icon , or select Normalize from the Data menu, or rightclick the item and select Normalize from the shortcut menu. The first Normalization dialog is displayed. 3. Double-click the Positive and Negative Control Genes radio button, or click it and click Next. The second Normalization dialog is displayed. GeneLinker Gold 3.1 / GeneLinker Platinum 2.1 270
