Download autoMACS® Pro Separator User manual

8 Place a wide mouth bottle under the column substitute.
Hold the column substitute in one hand and gently unscrew the
bottom column connector counter-clockwise. Unscrew the top
column connector while holding the column over the bottle to
catch any fluids. Store the column substitute in the autoMACS
Pro Separator – Starter Kit box.
Figure 3.26: Unscrewing the top column connector for column removal.
9 Insert one end of a fresh column into the bottom column
connector and gently screw in the column by turning it clockwise
until you feel a resistance. Point the column towards the top of
the instrument and screw in the top column connector.
10 Align the column with the top column connector sitting on the
guiding of the magnet cover. Press the column into the slot until
you feel the guides click. Verify that the column is placed in the
center of the magnet cover.
Note: The program will
then proceed to wash the
columns with autoMACS
Running Buffer. Check that
the columna are securely
fastened to the column
connectors and that no
buffer is leaking.
11 Repeat steps 8 through 11 to install the second column.
12 Ensure that the tubes are securely fastened to the columns and
that the tubing is neither pinched nor obstructed.
13 Press Done.
14 Close the front door. The unit is now ready to perform
cell separations.
3.3.5 Priming the autoMACS® Pro Separator
Priming implicates the initial cleaning and filling of the
autoMACS® Pro Separator tubing system before cell separations
are performed. The autoMACS Pro Separator must be primed
each time the instrument is switched ON.
WARNING! Read the warnings and precautions section before
priming the autoMACS Pro Separator for the first time.
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3 Assembly and installation